Rabbit anti-Human BAHD1 Polyclonal Antibody | anti-BAHD1 antibody

BAHD1, CT (BAHD1, KIAA0945, Bromo adjacent homology domain-containing 1 protein) (FITC)

Cat. Num. AAA6277562

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAHD1; Polyclonal Antibody; CT (BAHD1; KIAA0945; Bromo adjacent homology domain-containing 1 protein) (FITC); Bromo adjacent homology domain-containing 1 protein; anti-BAHD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BAHD1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751~780 amino acids from the C-terminal region of human BAHD1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BAHD1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BAHD1 antibody

BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.

Product Categories/Family for anti-BAHD1 antibody

Antibodies; KIAA; orf

References

Mayya V., et.al., Sci. Signal. 2:RA46-RA46(2009). Dephoure N., et.al., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 139394604
• NCBI GeneID: 22893
• NCBI Accession #: NP_055767.3
• NCBI GenBank Nucleotide #: NM_014952.3
• UniProt Accession #: Q8TBE0; Q8NDF7; Q9Y2F4
• Molecular Weight: 84,652 Da
• NCBI Official Full Name: bromo adjacent homology domain-containing 1 protein
• NCBI Official Synonym Full Names: bromo adjacent homology domain containing 1
• NCBI Official Symbol: BAHD1
• NCBI Protein Information: bromo adjacent homology domain-containing 1 protein; BAH domain-containing protein 1
• UniProt Protein Name: Bromo adjacent homology domain-containing 1 protein
• Protein Family: BAHD acyltransferase
• UniProt Gene Name: BAHD1
• UniProt Synonym Gene Names: KIAA0945; BAH domain-containing protein 1
• UniProt Entry Name: BAHD1_HUMAN

Uniprot Description

Function: Heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. May act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. Represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins. At specific stages of Listeria infection, in complex with TRIM28, corepresses interferon-stimulated genes, including IFNL1, IFNL2 and IFNL3. Ref.6 Ref.9

Subunit structure: Interacts with CBX5 (HP1 alpha), HDAC5, MBD1 and SP1. Forms a transcription silencing complex with at least CBX3 (HP1 gamma), HDAC1, HDAC2 and TRIM28. Interacts with L.monocytogenes LntA; this interaction, occurring at a late Listeria infection stage, relieves transcription repression, mostly that of interferon-stimulated genes. Ref.6 Ref.9

Subcellular location: Nucleus. Chromosome. Note: Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3). Ref.6

Domain: The BAH domain is required for localization at H3K27me3. Ref.6

Sequence similarities: Contains 1 BAH domain.

Sequence caution: The sequence BAA76789.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.