Rabbit anti-Human SF3B2 Polyclonal Antibody | anti-SF3B2 antibody

SF3B2, ID (SF3B2, SAP145, Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145kD subunit, SF3b150, Spliceosome-associated protein 145) (FITC)

Cat. Num. AAA6346540

• Gene Names: SF3B2; Cus1; SF3b1; SAP145; SF3B145; SF3b150
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SF3B2; Polyclonal Antibody; ID (SF3B2; SAP145; Splicing factor 3B subunit 2; Pre-mRNA-splicing factor SF3b 145kD subunit; SF3b150; Spliceosome-associated protein 145) (FITC); Spliceosome-associated protein 145; anti-SF3B2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SF3B2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SF3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 515-545 amino acids from the Central region of human SF3B2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SF3B2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SF3B2 antibody detected the SF3B2 protein (arrow).)

Related Product Information for anti-SF3B2 antibody

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq].

Product Categories/Family for anti-SF3B2 antibody

Antibodies; Splicing Factors

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Terada, Y., et al. Mol. Cell. Biol. 26(21):8149-8158(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 55749531
• NCBI GeneID: 10992
• NCBI Accession #: NP_006833.2
• NCBI GenBank Nucleotide #: NM_006842.2
• UniProt Accession #: Q13435; Q7L4T5; Q7Z627; Q969K1; Q96CM6; Q9BWD2; A8K485; B4DT19
• Molecular Weight: Predicted MW: 100kDa
• NCBI Official Full Name: splicing factor 3B subunit 2
• NCBI Official Synonym Full Names: splicing factor 3b subunit 2
• NCBI Official Symbol: SF3B2
• NCBI Official Synonym Symbols: Cus1; SF3b1; SAP145; SF3B145; SF3b150
• NCBI Protein Information: splicing factor 3B subunit 2
• UniProt Protein Name: High affinity choline transporter 1
• Protein Family: Splicing factor
• UniProt Gene Name: SLC5A7
• UniProt Synonym Gene Names: CHT1; CHT
• UniProt Entry Name: SC5A7_HUMAN

NCBI Description

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC5A7: Imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion- dependent. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: cell soma; integral to membrane; plasma membrane

Molecular Function: choline binding; choline transmembrane transporter activity; choline:sodium symporter activity

Biological Process: synaptic transmission; acetylcholine biosynthetic process; neurotransmitter secretion; sodium ion transport; neuromuscular synaptic transmission; choline transport; transmembrane transport; synaptic transmission, cholinergic

Disease: Neuronopathy, Distal Hereditary Motor, Type Viia