Western Blot (WB)
(Western blot analysis of anti-BACE Antibody (S498) in mouse cerebellum tissue lysates (35ug/lane). BACE (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse BACE Polyclonal Antibody | anti-BACE antibody
BACE (S498) (Beta-secretase 1, Beta-site APP Cleaving Enzyme 1, Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Aspartyl Protease 2, Asp 2, ASP2, Membrane-associated Aspartic Protease 2, Memapsin-2, BACE1, BACE, KIAA1149) (MaxLight 750)
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
BACE; Polyclonal Antibody; BACE (S498) (Beta-secretase 1; Beta-site APP Cleaving Enzyme 1; Beta-site Amyloid Precursor Protein Cleaving Enzyme 1; Aspartyl Protease 2; Asp 2; ASP2; Membrane-associated Aspartic Protease 2; Memapsin-2; BACE1; KIAA1149) (MaxLight 750); anti-BACE antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BACE. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-BACE antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding S498 of human BACE (KLH).
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-BACE Antibody (S498) in mouse cerebellum tissue lysates (35ug/lane). BACE (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-BACE Antibody (S498) in mouse cerebellum tissue lysates (35ug/lane). BACE (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-BACE antibody
References
1. Xie, J., et al., J. Biol. Chem. 280(14):13824-13832 (2005). 2. He, X., et al., J. Biol. Chem. 280(12):11696-11703 (2005). 3. Huang, X.P., et al., J. Biol. Chem. 279(36):37886-37894 (2004). 4. Chiocco, M.J., et al., J. Biol. Chem. 279(50):52535-52542 (2004). 5. Yang, H.C., et al., J. Neurochem. 91(6):1249-1259 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,107 Da
NCBI Official Full Name
beta-secretase 1 isoform A preproprotein
NCBI Official Synonym Full Names
beta-site APP-cleaving enzyme 1
NCBI Official Symbol
BACE1
NCBI Official Synonym Symbols
ASP2; BACE; HSPC104
NCBI Protein Information
beta-secretase 1; APP beta-secretase; asp 2; aspartyl protease 2; beta-secretase 1 precursor variant 1; beta-site APP cleaving enzyme 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; memapsin-2; membrane-associated aspartic protease 2; tran
UniProt Protein Name
Beta-secretase 1
Protein Family
UniProt Gene Name
BACE1
UniProt Synonym Gene Names
BACE; KIAA1149; ASP2; Asp 2
UniProt Entry Name
BACE1_HUMAN
Uniprot Description
BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.
Protein type: Protease; EC 3.4.23.46; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.2-q23.3
Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome
Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity
Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis
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Product Notes
The BACE bace1 (Catalog #AAA6464638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACE (S498) (Beta-secretase 1, Beta-site APP Cleaving Enzyme 1, Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Aspartyl Protease 2, Asp 2, ASP2, Membrane-associated Aspartic Protease 2, Memapsin-2, BACE1, BACE, KIAA1149) (MaxLight 750) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BACE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BACE bace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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