Human Interleukin 11 (IL11) ELISA Kit | IL11 elisa kit

Human Interleukin 11 (IL11) ELISA Kit

Cat. Num. AAA455471

• Gene Names: IL11; AGIF; IL-11
• Reactivity: Human
• Synonyms: Interleukin 11 (IL11); Human Interleukin 11 (IL11) ELISA Kit; AGIF Adipogenesis Inhibitory Factor Oprelvekin; IL11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL11. No significant cross-reactivity or interference between IL11 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.8pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL11 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL11 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL11 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL11 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10834994
• NCBI GeneID: 3589
• NCBI Accession #: NP_000632.1
• NCBI GenBank Nucleotide #: NM_000641.3
• UniProt Accession #: P20809; Q96EB4; B4DQV5
• Molecular Weight: 12,994 Da
• NCBI Official Full Name: interleukin-11 isoform 1
• NCBI Official Synonym Full Names: interleukin 11
• NCBI Official Symbol: IL11
• NCBI Official Synonym Symbols: AGIF; IL-11
• NCBI Protein Information: interleukin-11
• UniProt Protein Name: Interleukin-11
• Protein Family: Interleukin
• UniProt Gene Name: IL11
• UniProt Synonym Gene Names: AGIF

NCBI Description

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation (PubMed:12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3.