Mouse EPO ELISA Kit | EPO elisa kit

Mouse EPO PicoKine ELISA Kit

Cat. Num. AAA175795

• Reactivity: Mouse
• Synonyms: EPO; Mouse EPO PicoKine ELISA Kit; Erythropoietin; erythropoietin; EP; EPO_HUMAN; Epoetin; MVCD2; EPO elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse EPO
• Sequence Length: 192

• Samples: Cell culture supernates, serum, plasma (heparin) and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 46.9pg/ml-3000pg/ml
• Sensitivity: < 15pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse EPO PicoKine ELISA Kit standard curve )

Related Product Information for EPO elisa kit

Principle of the assay: mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards (NSO,A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.
Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

NCBI and Uniprot Product Information

• NCBI GI #: 119528
• NCBI GeneID: 13856
• UniProt Accession #: P07321
• Molecular Weight: 21,365 Da
• NCBI Official Full Name: Erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_MOUSE

NCBI Description

This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; heart morphogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; negative regulation of myeloid cell apoptosis; peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of protein kinase activity; positive regulation of cell proliferation; cardiac muscle morphogensis; response to hypoxia; erythrocyte differentiation; ventricular cardiac muscle morphogenesis; angiogenesis; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; negative regulation of apoptosis