Rabbit BACE Polyclonal Antibody | anti-BACE antibody

BACE (D492) Polyclonal Antibody

Cat. Num. AAA3000972

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BACE; Polyclonal Antibody; BACE (D492) Polyclonal Antibody; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2; BACE1; KIAA1149; anti-BACE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BACE (D492) polyclonal antibody detects endogenous levels of BACE protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 401

• Applicable Applications for anti-BACE antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 480-520 of Human BACE.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of BACE (D492) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-BACE antibody

Autosomal dominant Alzheimer's disease is caused by mutations in the gene encoding the beta-amyloid protein precursor (APP). Amyloid beta-peptide(Abeta), the major feature of amyloid plaques in Alzheimer's patients, is the product of APP cleavage by beta- and gamma-secretases. BACE is the transmembrane protease which cleaves Abeta from APP. BACE and the related protein Asp1 are both widely expressed in human tissue with the highest levels in the pancreas. BACE has a molecular mass of ~70 kDa, larger than predicted due to glycosylation, and is localized within Golgi and endosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 333440466
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193977.1
• NCBI GenBank Nucleotide #: NP_001193977.1
• UniProt Accession #: P56817
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: beta-secretase 1 isoform E
• NCBI Official Synonym Full Names: beta-secretase 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis