Rabbit anti-Human, Mouse BACE, Asp2 Polyclonal Antibody | anti-BACE1 antibody

BACE, Asp2 (CT) (Beta-site APP Cleaving Enzyme, Aspartyl Protease 2)

Cat. Num. AAA619573

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Highly Purified; Supplied as chromatographically purified IgG
• Synonyms: BACE; Asp2; Polyclonal Antibody; Asp2 (CT) (Beta-site APP Cleaving Enzyme; Aspartyl Protease 2); Anti -BACE; anti-BACE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human and mouse BACE/Asp2 (CT)
• Purity/Purification: Highly Purified; Supplied as chromatographically purified IgG
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-BACE1 antibody: Western Blot (WB)
• Immunogen: Peptide corresponding to amino acids 485 to 501 of human BACE (1).
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemicalanalysis of MBS619573.)

Western Blot (WB)

(Western Blot analysis of MBS619573.)

Related Product Information for anti-BACE1 antibody

Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b-and g-sites by two proteases. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4kD amyloid-b peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP.

This antibody can be used for detection of BACE by Western blot at 0.5 to 1ug/ml. Human brain tissue lysate can be used as positive control and an approximately 70kD band should be detected. It is human and mouse reactive

Product Categories/Family for anti-BACE1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 21040368
• NCBI GeneID: 23621
• NCBI Accession #: NP_620429.1
• NCBI GenBank Nucleotide #: NM_138973.3
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 55,711 Da
• NCBI Official Full Name: beta-secretase 1 isoform D preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; axon; endoplasmic reticulum; integral to plasma membrane; late endosome; plasma membrane; integral to membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis