IFNGR1 active protein

IFNGR1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120207

• Gene Names: IFNGR1; CD119; IFNGR
• Purity: >97% as determined by SDS-PAGE
• Synonyms: IFNGR1; IFNGR1 Protein; Human; Recombinant (His Tag); Human IFNGR1/CD119 Protein (His Tag); CD119 Protein; IFNGR Protein; IMD27A Protein; IMD27B Protein; interferon gamma receptor 1; IFNGR1 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly245

• Species: Human
• Activity: Measured by its ability to inhibit rhIFN-gamma mediated protection of WISH cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8ug/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Gly 245) of human IFN-gammaR1 pre-protein (NP_000407.1) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit rhIFN-gamma mediated protection of WISH cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8 ug/mL.)

Related Product Information for IFNGR1 active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 (interferon-gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interferon receptor while the IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-gamma was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-gamma exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12Novick D, et al. (1987) The human interferon-gamma receptor. The journal of biological chemistry. 262 (18): 8483-7

NCBI and Uniprot Product Information

• NCBI GI #: 4557880
• NCBI GeneID: 3459
• NCBI Accession #: NP_000407.1
• NCBI GenBank Nucleotide #: NM_000416.2
• UniProt Accession #: P15260; Q53Y96; E1P587
• Molecular Weight: 54,405 Da
• NCBI Official Full Name: interferon gamma receptor 1
• NCBI Official Synonym Full Names: interferon gamma receptor 1
• NCBI Official Symbol: IFNGR1
• NCBI Official Synonym Symbols: CD119; IFNGR
• NCBI Protein Information: interferon gamma receptor 1; CDw119; AVP, type 2; IFN-gamma-R1; CD119 antigen; IFN-gamma receptor 1; antiviral protein, type 2; immune interferon receptor 1; interferon-gamma receptor alpha chain
• UniProt Protein Name: Interferon gamma receptor 1
• Protein Family: Interferon gamma receptor
• UniProt Gene Name: IFNGR1
• UniProt Synonym Gene Names: IFN-gamma receptor 1
• UniProt Entry Name: INGR1_HUMAN

NCBI Description

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IFNGR1: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type II cytokine receptor family.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: endoplasmic reticulum; integral to plasma membrane; dendrite; postsynaptic density; integral to membrane; plasma membrane; vesicle

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interferon-gamma receptor activity; cytokine binding

Biological Process: cytokine and chemokine mediated signaling pathway; response to virus; signal transduction

Disease: Helicobacter Pylori Infection, Susceptibility To; Immunodeficiency 27b; Immunodeficiency 27a; Mycobacterium Tuberculosis, Susceptibility To; Hepatitis B Virus, Susceptibility To