Western Blot (WB)
(CAPN8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CAPN8 antibody detected the CAPN8 protein (arrow).)
Rabbit anti-Human CAPN8 Polyclonal Antibody | anti-CAPN8 antibody
CAPN8 Antibody (Center)
Gene Names
CAPN8; nCL-2
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CAPN8; Polyclonal Antibody; CAPN8 Antibody (Center); Calpain-8; New calpain 2; nCL-2; Stomach-specific M-type calpain; NCL2; anti-CAPN8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CAPN8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the Central region of human CAPN8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.38 (varies by lot)
Sequence Positions
290-318
Sequence Length
703
Applicable Applications for anti-CAPN8 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(CAPN8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CAPN8 antibody detected the CAPN8 protein (arrow).)
Western Blot (WB)
(CAPN8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CAPN8 antibody detected the CAPN8 protein (arrow).)
Immunohistochemistry (IHC)
(CAPN8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAPN8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CAPN8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAPN8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CAPN8 antibody
Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).
Product Categories/Family for anti-CAPN8 antibody
References
Hata, S., et al. J. Biol. Chem. 282(38):27847-27856(2007)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Hata, S., et al. J. Mol. Evol. 53(3):191-203(2001)
Huang, Y., et al. Trends Mol Med 7(8):355-362(2001)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Hata, S., et al. J. Mol. Evol. 53(3):191-203(2001)
Huang, Y., et al. Trends Mol Med 7(8):355-362(2001)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79144
NCBI Official Full Name
calpain-8
NCBI Official Synonym Full Names
calpain 8
NCBI Official Symbol
CAPN8
NCBI Official Synonym Symbols
nCL-2
NCBI Protein Information
calpain-8
UniProt Protein Name
Calpain-8
Protein Family
UniProt Gene Name
CAPN8
UniProt Synonym Gene Names
NCL2; nCL-2
UniProt Entry Name
CAN8_HUMAN
Uniprot Description
CAPN8: Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta- subunit of coatomer complex. Belongs to the peptidase C2 family.
Protein type: Motility/polarity/chemotaxis; EC 3.4.22.53; Protease
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: Golgi apparatus; cytoplasm
Molecular Function: calcium-dependent cysteine-type endopeptidase activity; calcium ion binding
Biological Process: digestion; proteolysis
Research Articles on CAPN8
Similar Products
Product Notes
The CAPN8 capn8 (Catalog #AAA9206911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 290-318. The CAPN8 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAPN8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the CAPN8 capn8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPN8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
