Western Blot (WB)
(Anti-MLK3 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa)
Rabbit anti-Human MLK3 Polyclonal Antibody | anti-MAP3K11 antibody
Anti-MLK3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MLK3; Polyclonal Antibody; Anti-MLK3 Antibody; Rabbit Polyclonal; MLK3/MAP3K11 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-MEKK11 Antibody; Anti-MLK-3 Antibody; Anti-PTK1 Antibody; Anti-SPRK Antibody; mitogen-activated protein kinase kinase kinase 11; anti-MAP3K11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MLK3/MAP3K11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MAP3K11 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human MLK3/MAP3K11 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MLK3/MAP3K11 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Western Blot (WB)
(Anti-MLK3 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa)
Western Blot (WB)
(Anti-MLK3 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa)
Related Product Information for anti-MAP3K11 antibody
Background: Mixed-lineage kinase 3 (MLK3; also known as MAP3K11) is a Ser/Thr protein kinase widely expressed in normal and cancerous tissues, including brain, lung, liver, heart, and skeletal muscle tissues. Its Src homology 3 (SH3) domain has been implicated in MLK3 autoinhibition and interactions with other proteins, including those from viruses. The MLK3 SH3 domain contains a six-amino-acid insert corresponding to the n-Src insert, suggesting that MLK3 may bind additional peptides. MAP3K11 was initially identified in a megakaryocytic cell line and is an emerging therapeutic target in cancer. MAP3K11 directly phosphorylates JNKs and may control activation of ERKs. MAP3K11 is a tightly regulated MAP3K member. Consistent with its known role in the activation of the AP-1 pathway through JNK kinase, MLK3 was able to enhance Tat-dependent HIV transcription in vitro thus leading to an increase in infection signal.
Product Categories/Family for anti-MAP3K11 antibody
References
Nguyen DG, et al. (2007) Identification of novel therapeutic targets for hiv infection through functional genomic cdna screening. Virology 362 (1): 16-25.Kokoszka ME, et al. (2018) Identification of two distinct peptide-binding pockets in the sh3 domain of human mixed-lineage kinase 3. J Biol Chem 293 (35): 13553-13565.Gibbs GM, et al. (2007) Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm. Biol Reprod 77 (1): 108-114.
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Product Notes
The MAP3K11 (Catalog #AAA8119387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MLK3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MAP3K11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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