Goat AXIN1 / Axin-1 Polyclonal Antibody | anti-AXIN1 antibody

Goat Polyclonal to Human AXIN1 / Axin-1

Cat. Num. AAA242192

• Gene Names: AXIN1; AXIN; PPP1R49
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Zebrafish
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: AXIN1 / Axin-1; Polyclonal Antibody; Goat Polyclonal to Human AXIN1 / Axin-1; Anti-AXIN1 / Axin-1 Antibody (C-Terminus) IHC-plus; AVPR2; ADHR; AVPR V2; Avp2r; Antidiuretic hormone receptor; DI1; DIR3; NDI; Vasopressin type 2 receptor; Vasopressin type II receptor; Vasopressin V2 receptor; V2 vasopressin receptor; Vasopressin receptor type 2; DIR; Type 2 vasopressin receptor; Human AXIN1; Axin-1; anti-AXIN1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Zebrafish
• Clonality: Polyclonal
• Specificity: Human AXIN1. This antibody is expected to recognise both reported isoforms.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Positions: 850-862

• Applicable Applications for anti-AXIN1 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IHC - Paraffin (3.75ug/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
• Target: AXIN / Axin-1
• Immunogen species: AXIN / Axin-1 antibody was raised againist Human
• Immunogen: AXIN / Axin-1 antibody was raised against peptide with sequence C-FEEKIIGKVEKVD, from the C-Terminus of protein sequence accordinf=g to NP_003493, NP_851393.1.
• Usage: Immunohistochemistry: MBS242192 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242192 was determined to be 3.75 ug/ml.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-AXIN1 / AXIN antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 27501450
• NCBI GeneID: 8312
• NCBI Accession #: NP_003493.1
• NCBI GenBank Nucleotide #: NM_003502.3
• UniProt Accession #: O15169; Q4TT26; Q4TT27; Q86YA7; Q8WVW6; Q96S28
• NCBI Official Full Name: axin-1 isoform a
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; PPP1R49
• NCBI Protein Information: axin-1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1; protein phosphatase 1, regulatory subunit 49
• UniProt Protein Name: Axin-1
• Protein Family: Axin
• UniProt Gene Name: AXIN1
• UniProt Synonym Gene Names: AXIN; hAxin
• UniProt Entry Name: AXIN1_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasmic membrane-bound vesicle; postsynaptic density; cytoplasm; cytoplasmic vesicle; cell cortex; beta-catenin destruction complex; cytosol; nucleus; lateral plasma membrane

Molecular Function: protein C-terminus binding; identical protein binding; signal transducer activity; protein binding; protein homodimerization activity; receptor signaling complex scaffold activity; enzyme binding; protein self-association; p53 binding; ubiquitin protein ligase binding; beta-catenin binding; protein complex scaffold; SMAD binding; protein kinase binding; GTPase activator activity; receptor binding

Biological Process: cell death; protein polyubiquitination; negative regulation of Wnt receptor signaling pathway; apoptosis; embryonic eye morphogenesis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of protein metabolic process; nucleocytoplasmic transport; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; activation of JNK activity; cytoplasmic microtubule organization and biogenesis; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; positive regulation of protein catabolic process; activation of protein kinase activity; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity

Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma