Rabbit anti-Mouse Aurka Polyclonal Antibody | anti-Aurka antibody

Aurka, NT (Aurka, Aik, Airk, Ark1, Aura, Ayk1, Btak, Iak1, Stk15, Stk6, Aurora kinase A, Aurora 2, Aurora family kinase 1, Aurora/IPL1-related kinase 1, Ipl1-and aurora-related kinase 1, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase A

Cat. Num. AAA6276855

• Gene Names: Aurka; IAK; Ark1; Ayk1; IAK1; Stk6; AIRK1; AU019385; AW539821; Aurora-A
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Aurka; Polyclonal Antibody; NT (Aurka; Aik; Airk; Ark1; Aura; Ayk1; Btak; Iak1; Stk15; Stk6; Aurora kinase A; Aurora 2; Aurora family kinase 1; Aurora/IPL1-related kinase 1; Ipl1-and aurora-related kinase 1; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase A; Serine/threonine-protein kinase Ayk1; Serine/threonine-protein kinase aurora-A; anti-Aurka antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Aurka antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Aurka antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-120 amino acids from the N-terminal region of Mouse Aurka.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Aurka Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Aurka antibody detected the Aurka protein (arrow).)

Related Product Information for anti-Aurka antibody

Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Product Categories/Family for anti-Aurka antibody

Antibodies; Protein Kinases

References

Kinzel, D., et al. Dev. Cell 19(1):66-77(2010). Van Horn, R.D., et al. J. Biol. Chem. 285(28):21849-21857(2010). Mori, D., et al. Nat. Cell Biol. 11(9):1057-1068(2009). Li, C.C., et al. Mol. Cancer Res. 7(5):678-688(2009). Tseng, Y.S., et al. BMC Cancer 9, 435 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 46358064
• NCBI GeneID: 20878
• NCBI Accession #: NP_035627.1
• NCBI GenBank Nucleotide #: NM_011497.3
• UniProt Accession #: P97477; O35624; Q8C3H8; Q91YU4
• Molecular Weight: 44,772 Da
• NCBI Official Full Name: aurora kinase A
• NCBI Official Synonym Full Names: aurora kinase A
• NCBI Official Symbol: Aurka
• NCBI Official Synonym Symbols: IAK; Ark1; Ayk1; IAK1; Stk6; AIRK1; AU019385; AW539821; Aurora-A
• NCBI Protein Information: aurora kinase A; ARK-1; aurora 2; aurora A; aurora family kinase 1; aurora-related kinase 1; serine/threonine kinase 6; aurora/IPL1-related kinase 1; ipl1- and aurora-related kinase 1; serine/threonine-protein kinase 6; serine/threonine-protein kinase Ayk
• UniProt Protein Name: Aurora kinase A
• Protein Family: Aurora kinase
• UniProt Gene Name: Aurka
• UniProt Synonym Gene Names: Aik; Airk; Ark1; Aura; Ayk1; Btak; Iak1; Stk15; Stk6; ARK-1; Aurora-related kinase 1
• UniProt Entry Name: AURKA_MOUSE

Uniprot Description

AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; AUR family

Cellular Component: germinal vesicle; centrosome; microtubule; pronucleus; spindle pole centrosome; axon hillock; microtubule cytoskeleton; cytoskeleton; spindle microtubule; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; spindle; spindle midzone; nucleus; condensed nuclear chromosome, pericentric region

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; nucleotide binding; histone serine kinase activity; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: spindle stabilization; meiosis; mitosis; regulation of protein stability; microtubule cytoskeleton organization and biogenesis; cell cycle; regulation of cytokinesis; protein amino acid phosphorylation; mitotic centrosome separation; mitotic spindle organization and biogenesis; cell projection organization and biogenesis; centrosome localization; cell division; centrosome organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; negative regulation of protein binding; phosphorylation; spindle assembly involved in female meiosis I; meiotic spindle organization and biogenesis; negative regulation of apoptosis; anterior/posterior axis specification