Western Blot (WB)
(Western Blot analysis of GALNT14 expression in transfected 293T cell line by GALNT14 polyclonal antibody. Lane 1: GALNT14 transfected lysate (60.72kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human GALNT14 Polyclonal Antibody | anti-GALNT14 antibody
GALNT14 (Polypeptide N-acetylgalactosaminyltransferase 14, Polypeptide GalNAc Transferase 14, Protein-UDP acetylgalactosaminyltransferase 14, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 14, UNQ2434/PRO4994, FLJ12691, FLJ13977)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of GALNT14 expression in transfected 293T cell line by GALNT14 polyclonal antibody. Lane 1: GALNT14 transfected lysate (60.72kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of GALNT14 expression in transfected 293T cell line by GALNT14 polyclonal antibody. Lane 1: GALNT14 transfected lysate (60.72kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a Golgi protein which is a member of the polypeptide N-acetylgalactosaminyltransferase (ppGalNAc-Ts) protein family. These enzymes catalyze the transfer of N-acetyl-D-galactosamine (GalNAc) to the hydroxyl groups on serines and threonines in target peptides. The encoded protein has been shown to transfer GalNAc to large proteins like mucins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays activity toward mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). May be involved in O-glycosylation in kidney.
Catalytic activity: UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide. Ref.1
Cofactor: Manganese
By similarity.Calcium
By similarity.
Pathway: Protein modification; protein glycosylation.
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
By similarity.
Tissue specificity: Detected in renal tubules (at protein level). Highly expressed in fetal and adult kidney. Widely expressed at low level. Weakly expressed in whole brain, cerebellum, thymus, lung, mammary gland, liver, stomach, small intestine, colon, pancreas, spleen, bladder, uterus, placenta, testis, ovary, skeletal muscle, leukocyte, B-cell, bone marrow, fetal brain, fetal thymus, fetal lung, fetal liver, fetal small intestine, fetal spleen, fetal skeletal and fetus. Detected in renal tubules (at protein level). Ref.1 Ref.8
Domain: There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding
By similarity.The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity
By similarity.
Sequence similarities: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.Contains 1 ricin B-type lectin domain.
Sequence caution: The sequence BAB14795.1 differs from that shown. Reason: Chimeric at the C-terminus.
Research Articles on GALNT14
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Product Notes
The GALNT14 galnt14 (Catalog #AAA649827) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GALNT14 (Polypeptide N-acetylgalactosaminyltransferase 14, Polypeptide GalNAc Transferase 14, Protein-UDP acetylgalactosaminyltransferase 14, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 14, UNQ2434/PRO4994, FLJ12691, FLJ13977) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the GALNT14 galnt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRRLTRRLVL PVFGVLWITV LLFFWVTKRK LEVPTGPEVQ TPKPSDADWD DLWDQFDERR YLNAKKWRVG DDPYKLYAFN QRESERISSN RAIPDTRHLR CTLLVYCTDL PPTSIIITFH NEARSTLLRT IRSVLNRTPT HLIREIILVD DFSNDPDDCK QLIKLPKVKC LRNNERQGLV RSRIRGADIA QGTTLTFLDS HCEVNRDWLQ PLLHRVKEDY TRVVCPVIDI INLDTFTYIE SASELRGGFD WSLHFQWEQL SPEQKARRLD PTEPIRTPII AGGLFVIDKA WFDYLGKYDM DMDIWGGENF EISFRVWMCG GSLEIVPCSR VGHVFRKKHP YVFPDGNANT YIKNTKRTAE VWMDEYKQYY YAARPFALER PFGNVESRLD LRKNLRCQSF KWYLENIYPE LSIPKESSIQ KGNIRQRQKC LESQRQNNQE TPNLKLSPCA KVKGEDAKSQ VWAFTYTQQI LQEELCLSVI TLFPGAPVVL VLCKNGDDRQ QWTKTGSHIE HIASHLCLDT DMFGDGTENG KEIVVNPCES SLMSQHWDMV SS. It is sometimes possible for the material contained within the vial of "GALNT14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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