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Western Blot (WB) (Western Blot: Sample: Mouse Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse ATP1b3 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Rabbit anti-Mouse ATPase Polyclonal Antibody | anti-ATP1b3 antibody

Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)

Gene Names
Atp1b3; AA409958; AI664000; AW212096
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
ATPase; Polyclonal Antibody; Polyclonal Antibody to ATPase; Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3); anti-ATP1b3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ATP1b3. It has beenselected for its ability to recognize ATP1b3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MLQTLNDEVP KYRDQIPSPG LMVFPKPQTA LEYTFSMSEP QTYKKLVEDL ESFLKPYSVE EQKNLTSCPD GAPFIQHGPD YRACQFPVSL LEECSGVTDA NFGYSKGQPC ILVKMNRIID LIPDGYPQIS CLPKEENATI ATYPEFGVLD LKYFPYYGKK RHVGYRQPLV AVQVKFDSGL NKKEVTVECH IAGTRNLKNK NERDKFLGRV SFKVTAR
Sequence Length
1115
Applicable Applications for anti-ATP1b3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Fragment
ATP1b3 (Met61~Arg277)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant ATP1b3 (Met61~Arg277) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2046504
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse ATP1b3 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB) (Western Blot: Sample: Mouse Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse ATP1b3 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded skeletal muscle tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded skeletal muscle tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,776 Da
NCBI Official Full Name
sodium/potassium-transporting ATPase subunit beta-3 isoform 1
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, beta 3 polypeptide
NCBI Official Symbol
Atp1b3
NCBI Official Synonym Symbols
AA409958; AI664000; AW212096
NCBI Protein Information
sodium/potassium-transporting ATPase subunit beta-3
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit beta-3
Protein Family
UniProt Gene Name
Atp1b3
UniProt Synonym Gene Names
ATPB-3

Uniprot Description

ATP1B3: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.

Protein type: Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 9 E3.3|9 50.31 cM

Cellular Component: caveola; cytoplasm; extracellular exosome; integral component of membrane; membrane; plasma membrane; sodium:potassium-exchanging ATPase complex

Molecular Function: ATPase activator activity; ATPase binding; protein binding; sodium:potassium-exchanging ATPase activity

Biological Process: cellular potassium ion homeostasis; cellular sodium ion homeostasis; ion transport; membrane repolarization; metal ion transport; positive regulation of ATPase activity; positive regulation of potassium ion transmembrane transporter activity; potassium ion import; potassium ion transport; protein localization to plasma membrane; protein stabilization; sodium ion transport; transport

Research Articles on ATP1b3

Similar Products

Product Notes

The ATP1b3 atp1b3 (Catalog #AAA2027286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL;1:50-200 Immunocytochemistry: 5-20ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ATP1b3 atp1b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MLQTLNDEVP KYRDQIPSPG LMVFPKPQTA LEYTFSMSEP QTYKKLVEDL ESFLKPYSVE EQKNLTSCPD GAPFIQHGPD YRACQFPVSL LEECSGVTDA NFGYSKGQPC ILVKMNRIID LIPDGYPQIS CLPKEENATI ATYPEFGVLD LKYFPYYGKK RHVGYRQPLV AVQVKFDSGL NKKEVTVECH IAGTRNLKNK NERDKFLGRV SFKVTAR. It is sometimes possible for the material contained within the vial of "ATPase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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