Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse Smad4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Mothers Against Decapentaplegic Homolog 4 Polyclonal Antibody | anti-Smad4 antibody
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSMLVKDE YVHDFEGQPS LPTEGHSIQT IQHPPSNRAS TETYSAPALL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse Smad4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse Smad4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue))
NCBI and Uniprot Product Information
Uniprot Description
SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.
Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 18 E2|18 49.51 cM
Cellular Component: centrosome; cytoplasm; cytosol; intracellular; nuclear chromatin; nucleoplasm; nucleus; protein complex; RNA polymerase II transcription factor complex; SMAD protein complex; transcription factor complex
Molecular Function: chromatin binding; collagen binding; DNA binding; DNA binding transcription factor activity; filamin binding; I-SMAD binding; identical protein binding; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; R-SMAD binding; sequence-specific DNA binding; SMAD binding; sulfate binding; transcription regulatory region DNA binding; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
Biological Process: anterior/posterior pattern specification; atrioventricular valve formation; axon guidance; BMP signaling pathway; brainstem development; branching involved in ureteric bud morphogenesis; cardiac septum development; cell proliferation; cellular iron ion homeostasis; cellular response to glucose stimulus; developmental growth; embryonic digit morphogenesis; endoderm development; endothelial cell activation; epithelial to mesenchymal transition involved in endocardial cushion formation; ERK1 and ERK2 cascade; female gonad development; formation of anatomical boundary; gastrulation; gastrulation with mouth forming second; in utero embryonic development; interleukin-6-mediated signaling pathway; kidney development; left ventricular cardiac muscle tissue morphogenesis; male gonad development; mesoderm development; metanephric mesenchyme morphogenesis; negative regulation of cardiac muscle hypertrophy; negative regulation of cardiac myofibril assembly; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of ERK1 and ERK2 cascade; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nephrogenic mesenchyme morphogenesis; neural crest cell differentiation; neuron fate commitment; osteoblast differentiation; outflow tract septum morphogenesis; ovarian follicle development; palate development; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell proliferation involved in heart valve morphogenesis; positive regulation of epithelial to mesenchymal transition; positive regulation of follicle-stimulating hormone secretion; positive regulation of histone H3-K4 methylation; positive regulation of histone H3-K9 acetylation; positive regulation of luteinizing hormone secretion; positive regulation of pathway-restricted SMAD protein phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of binding; regulation of cell proliferation; regulation of hair follicle development; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; response to transforming growth factor beta; sebaceous gland development; seminiferous tubule development; single fertilization; SMAD protein complex assembly; somite rostral/caudal axis specification; spermatogenesis; tissue morphogenesis; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; uterus development; wound healing
Research Articles on Smad4
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Product Notes
The Smad4 smad4 (Catalog #AAA2028116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mothers Against Decapentaplegic Homolog 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the Smad4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSMLVKDE YVHDFEGQPS LPTEGHSIQT IQHPPSNRAS TETYSAPALL. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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