Western Blot (WB)
(Western blot analysis of ATP6V1H Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).ATP6V1H (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ATP6V1H Polyclonal Antibody
ATP6V1H, CT (ATP6V1H, V-type proton ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57kD subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ATP6V1H; Polyclonal Antibody; CT (ATP6V1H; V-type proton ATPase subunit H; Nef-binding protein 1; Protein VMA13 homolog; V-ATPase 50/57kD subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD); Anti -ATP6V1H; anti-ATP6V1H antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ATP6V1H antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
ATP6V1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-437 amino acids from the C-terminal region of human ATP6V1H.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ATP6V1H Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).ATP6V1H (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ATP6V1H Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).ATP6V1H (arrow) was detected using the purified Pab.)
Related Product Information for anti-ATP6V1H antibody
ATP6V1H encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase.
Product Categories/Family for anti-ATP6V1H antibody
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Product Notes
The ATP6V1H (Catalog #AAA647643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP6V1H, CT (ATP6V1H, V-type proton ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57kD subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6V1H can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the ATP6V1H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6V1H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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