Rabbit ATP6V1H Polyclonal Antibody | anti-ATP6V1H antibody

ATP6V1H Antibody (C-term)

Cat. Num. AAA9214377

• Gene Names: ATP6V1H; SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Pig)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ATP6V1H; Polyclonal Antibody; ATP6V1H Antibody (C-term); V-type proton ATPase subunit H; V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; anti-ATP6V1H antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Pig)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ATP6V1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-426 amino acids from the C-terminal region of human ATP6V1H.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 400-426
• Sequence Length: 465

• Applicable Applications for anti-ATP6V1H antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ATP6V1H Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).ATP6V1H (arrow) was detected using the purified Pab.)

Related Product Information for anti-ATP6V1H antibody

ATP6V1H encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase.

Product Categories/Family for anti-ATP6V1H antibody

Metabolism; Signal Transduction

References

Fuster, D.G., et al. Kidney Int. 73(10):1151-1158(2008)
Stove, V., et al. J. Virol. 79(17):11422-11433(2005)
Morel, N. Biol. Cell 95(7):453-457(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 47717104
• NCBI GeneID: 51606
• NCBI Accession #: NP_057025.2; NP_998784.1; NP_998785.1
• NCBI GenBank Nucleotide #: NM_015941.3
• UniProt Accession #: Q9UI12; Q6PK44; Q9H3E3; Q9Y300; B3KMR0
• Molecular Weight: 55883
• NCBI Official Full Name: V-type proton ATPase subunit H isoform 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• NCBI Official Symbol: ATP6V1H
• NCBI Official Synonym Symbols: SFD; NBP1; VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
• NCBI Protein Information: V-type proton ATPase subunit H
• UniProt Protein Name: V-type proton ATPase subunit H
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1H
• UniProt Synonym Gene Names: V-ATPase subunit H; NBP1
• UniProt Entry Name: VATH_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

ATP6V1H: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. Belongs to the V-ATPase H subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 8q11.2

Cellular Component: lysosomal membrane; plasma membrane; vacuolar proton-transporting V-type ATPase, V1 domain; cytosol

Molecular Function: protein binding; ATPase activity; hydrogen ion transporting ATPase activity, rotational mechanism; enzyme regulator activity

Biological Process: interaction with host; vacuolar acidification; viral reproduction; metabolic process; cellular iron ion homeostasis; regulation of catalytic activity; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; endocytosis; transmembrane transport; regulation of defense response to virus by virus