Rabbit anti-Human, Mouse ATP6V1E1 Polyclonal Antibody | anti-ATP6V1E1 antibody

ATP6V1E1 Antibody

Cat. Num. AAA9414706

• Gene Names: ATP6V1E1; P31; Vma4; ATP6E; ATP6E2; ATP6V1E
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were purified by antigen-affinity chromatography.
• Synonyms: ATP6V1E1; Polyclonal Antibody; ATP6V1E1 Antibody; ATP6E antibody; ATP6E2 antibody; ATP6V1E antibody; P31 antibody; Vma4 antibody; ATP6V1E1 antibody; "H(+)-transporting two-sector ATPase; 31kDa subunit antibody"; "H+-transporting ATP synthase chain E; vacuolar antibody"; vacuolar proton pump subunit E 1 a; anti-ATP6V1E1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATP6V1E1 protein.
• Purity/Purification: Antibodies were purified by antigen-affinity chromatography.
• Form/Format: Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: 0.17 mg/ml (varies by lot)
• Sequence Length: 204

• Applicable Applications for anti-ATP6V1E1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500-1:3000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 1 and 226 of ATP6V1E1.
• Target Name: ATP6V1E1
• Preparation and Storage: Store at -20 degree C

Testing Data

(Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000)

Testing Data

(Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE diluted at 1:1000)

Related Product Information for anti-ATP6V1E1 antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Product Categories/Family for anti-ATP6V1E1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 87159816
• NCBI GeneID: 529
• NCBI Accession #: NP_001034455.1
• NCBI GenBank Nucleotide #: NM_001039366.1
• UniProt Accession #: P36543; A8MUE4; A8MUN4
• Molecular Weight: 22,706 Da
• NCBI Official Full Name: V-type proton ATPase subunit E 1 isoform b
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
• NCBI Official Symbol: ATP6V1E1
• NCBI Official Synonym Symbols: P31; Vma4; ATP6E; ATP6E2; ATP6V1E
• NCBI Protein Information: V-type proton ATPase subunit E 1
• UniProt Protein Name: V-type proton ATPase subunit E 1
• UniProt Gene Name: ATP6V1E1
• UniProt Synonym Gene Names: ATP6E; ATP6E2; V-ATPase subunit E 1; p31
• UniProt Entry Name: VATE1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1E1: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase E subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Mitochondrial

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: microvillus; mitochondrion; lysosomal membrane; apical plasma membrane; proton-transporting two-sector ATPase complex; cytosol; endosome

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: interaction with host; proton transport; metabolic process; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport