Rabbit ATP6V1E1 Polyclonal Antibody | anti-ATP6V1E1 antibody

ATP6V1E1 Rabbit Polyclonal

Cat. Num. AAA766954

• Gene Names: ATP6V1E1; P31; Vma4; ATP6E; ATP6E2; ATP6V1E
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ATP6V1E1; Polyclonal Antibody; ATP6V1E1 Rabbit Polyclonal; anti-ATP6V1E1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 226

• Applicable Applications for anti-ATP6V1E1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Testing Data

()

Immunoprecipitation (IP)

(IP Result of anti-ATP6V1E1 (IP: MBS766954, 4ug; Detection: MBS766954 1:500) with mouse brain tissue lysate 6000ug.)

Testing Data

()

NCBI and Uniprot Product Information

• NCBI GI #: 109451604
• NCBI GeneID: 529
• Molecular Weight: 22,706 Da
• NCBI Official Full Name: ATP6V1E1, partial
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit E1
• NCBI Official Symbol: ATP6V1E1
• NCBI Official Synonym Symbols: P31; Vma4; ATP6E; ATP6E2; ATP6V1E
• NCBI Protein Information: V-type proton ATPase subunit E 1
• UniProt Protein Name: V-type proton ATPase subunit E 1
• UniProt Gene Name: ATP6V1E1
• UniProt Synonym Gene Names: ATP6E; ATP6E2; V-ATPase subunit E 1; p31
• UniProt Entry Name: VATE1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1E1: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase E subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Mitochondrial; Hydrolase

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: apical plasma membrane; cytosol; endosome; lysosomal membrane; microvillus; mitochondrion; proton-transporting two-sector ATPase complex

Molecular Function: ATPase binding; hydrogen ion transporting ATPase activity, rotational mechanism; hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding

Biological Process: ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; metabolic process; proton transport; regulation of macroautophagy; transferrin transport