Western Blot (WB)
(HNRNPD Antibody (N-term) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).)
Rabbit HNRNPD Polyclonal Antibody | anti-HNRNPD antibody
HNRNPD Antibody (N-term)
Western Blot (WB)
(HNRNPD Antibody (N-term) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).)
Western Blot (WB)
(HNRNPD Antibody (N-term) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The
hnRNPs are nucleic acid binding proteins and they complex with
heterogeneous nuclear RNA (hnRNA). These proteins are associated
with pre-mRNAs in the nucleus and appear to influence pre-mRNA
processing and other aspects of mRNA metabolism and transport.
While all of the hnRNPs are present in the nucleus, some seem to
shuttle between the nucleus and the cytoplasm. The hnRNP proteins
have distinct nucleic acid binding properties. The protein encoded
by this gene has two repeats of quasi-RRM domains that bind to
RNAs. It localizes to both the nucleus and the cytoplasm. This
protein is implicated in the regulation of mRNA stability.
Alternative splicing of this gene results in four transcript
variants.
Zhai, B., et al. J. Biol. Chem. 285(31):23568-23580(2010)
Vazquez-Chantada, M., et al. Gastroenterology 138(5):1943-1953(2010)
Trojanowicz, B., et al. Endocr. Relat. Cancer 16(3):857-871(2009)
Pautz, A., et al. J. Biol. Chem. 284(5):2755-2766(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP D0: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). Implicated in the regulation of mRNA stability. Has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. Four alternatively spliced transcript variants have been described.
Protein type: RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: nucleoplasm; ribonucleoprotein complex; nucleus; cytosol
Molecular Function: protein binding; RNA binding; telomeric DNA binding; nucleotide binding
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; positive regulation of translation; regulation of transcription, DNA-dependent; RNA splicing; positive regulation of transcription, DNA-dependent; RNA catabolic process; gene expression; regulation of circadian rhythm; regulation of mRNA stability
Research Articles on HNRNPD
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Product Notes
The HNRNPD hnrnpd (Catalog #AAA9210846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-85. The HNRNPD Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HNRNPD can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the HNRNPD hnrnpd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNRNPD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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