Rabbit anti-Human TRIM25 Polyclonal Antibody | anti-TRIM25 antibody

TRIM25 (E3 Ubiquitin/ISG15 Ligase TRIM25, Estrogen-responsive Finger Protein, EFP, RNF147, ZNF147, RING Finger Protein 147, Tripartite Motif-containing Protein 25, Ubiquitin/ISG15-conjugating Enzyme TRIM25, Zinc Finger Protein 147) (HRP)

Cat. Num. AAA6397219

• Gene Names: TRIM25; EFP; Z147; RNF147; ZNF147
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM25; Polyclonal Antibody; TRIM25 (E3 Ubiquitin/ISG15 Ligase TRIM25; Estrogen-responsive Finger Protein; EFP; RNF147; ZNF147; RING Finger Protein 147; Tripartite Motif-containing Protein 25; Ubiquitin/ISG15-conjugating Enzyme TRIM25; Zinc Finger Protein 147) (HRP); anti-TRIM25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIM25.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 2445

• Applicable Applications for anti-TRIM25 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TRIM25, aa1-630 (AAH16924.1).
• Immunogen Sequence: MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRIM25 rabbit polyclonal antibody. Western Blot analysis of TRIM25 expression in human placenta.)

Western Blot (WB)

(Western Blot analysis of TRIM25 expression in transfected 293T cell line by TRIM25 polyclonal antibody. Lane 1: TRIM25 transfected lysate (71.0kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TRIM25 antibody

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo-or heterodimers, which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.

Product Categories/Family for anti-TRIM25 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 16877338
• NCBI GeneID: 7706
• NCBI Official Full Name: Homo sapiens tripartite motif-containing 25, mRNA
• NCBI Official Synonym Full Names: tripartite motif containing 25
• NCBI Official Symbol: TRIM25
• NCBI Official Synonym Symbols: EFP; Z147; RNF147; ZNF147
• NCBI Protein Information: E3 ubiquitin/ISG15 ligase TRIM25
• Protein Family: E3 ubiquitin/ISG15 ligase

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]