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Western Blot (WB) (Western blot analysis of ATP5L using COLO205 whole cell lysates)

Rabbit anti-Human, Mouse ATP5L Polyclonal Antibody | anti-ATP5L antibody

ATP5L Antibody

Gene Names
ATP5MG; ATP5L; ATP5JG
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ATP5L; Polyclonal Antibody; ATP5L Antibody; ATP synthase subunit g; ATP synthase; H+ transporting; mitochondrial F1F0; subunit g; mitochondrial Fo complex; subunit G; ATP5L_HUMAN; ATPase subunit g; F1Fo ATP synthase complex Fo membrane domain g subunit; mitochondrial; anti-ATP5L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ATP5L antibody detects endogenous levels of total ATP5L
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
103
Applicable Applications for anti-ATP5L antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ATP5L
Subcellular Location
Mitochondrion. Mitochondrion Inner Membrane.
Predicted Cross Reactivity
Zebrafish, Bovine
Similarity
Zebrafish (92%), Bovine (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ATP5L using COLO205 whole cell lysates)

Western Blot (WB) (Western blot analysis of ATP5L using COLO205 whole cell lysates)
Related Product Information for anti-ATP5L antibody
Function: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1-containing the extramembraneous catalytic core, and F0-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
Subunit Structure: F-type ATPases have 2 components, CF1-the catalytic core-and CF0-the membrane proton channel. CF0 seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5F1, ATP5MC1, ATP5F1E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5O, ATP5L, USMG5 and MP68 (By similarity).
Similarity: Belongs to the ATPase g subunit family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 11 kDa
Predicted: 12 kDa
NCBI Official Full Name
ATP synthase subunit g, mitochondrial
NCBI Official Synonym Full Names
ATP synthase membrane subunit g
NCBI Official Symbol
ATP5MG
NCBI Official Synonym Symbols
ATP5L; ATP5JG
NCBI Protein Information
ATP synthase subunit g, mitochondrial
UniProt Protein Name
ATP synthase subunit g, mitochondrial
Protein Family
UniProt Gene Name
ATP5MG
UniProt Synonym Gene Names
ATPase subunit g

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.

Research Articles on ATP5L

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Product Notes

The ATP5L atp5mg (Catalog #AAA9609558) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ATP5L atp5mg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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