Rabbit anti-Human ATP13A3 Polyclonal Antibody | anti-ATP13A3 antibody

ATP13A3, NT (ATP13A3, AFURS1, Probable cation-transporting ATPase 13A3, ATPase family homolog up-regulated in senescence cells 1) (FITC)

Cat. Num. AAA6276384

• Gene Names: ATP13A3; AFURS1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ATP13A3; Polyclonal Antibody; NT (ATP13A3; AFURS1; Probable cation-transporting ATPase 13A3; ATPase family homolog up-regulated in senescence cells 1) (FITC); ATPase family homolog up-regulated in senescence cells 1; anti-ATP13A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP13A3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ATP13A3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 101-129 in the N-terminal region of human ATP13A3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in HepG2 cell line lysates (35ug/lane), usingMBS6004535. This demonstrates that MBS6004535 detected the ATP13A3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma, using MBS6004535 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004535 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells (right histogram), using MBS6004535 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATP13A3 antibody

Antibodies; Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 148839292
• NCBI GeneID: 79572
• NCBI Accession #: NP_078800.3
• NCBI GenBank Nucleotide #: NM_024524.3
• UniProt Accession #: Q9H7F0; Q8NC11; Q96KS1
• Molecular Weight: 77,310 Da
• NCBI Official Full Name: probable cation-transporting ATPase 13A3
• NCBI Official Synonym Full Names: ATPase 13A3
• NCBI Official Symbol: ATP13A3
• NCBI Official Synonym Symbols: AFURS1
• NCBI Protein Information: probable cation-transporting ATPase 13A3
• UniProt Protein Name: Probable cation-transporting ATPase 13A3
• Protein Family: Probable cation-transporting ATPase
• UniProt Gene Name: ATP13A3
• UniProt Synonym Gene Names: AFURS1

NCBI Description

ATP13A3 is a member of the P-type ATPase family of proteins that transport a variety of cations across membranes. Other P-type ATPases include ATP7B (MIM 606882) and ATP7A (MIM 300011).[supplied by OMIM, Aug 2008]

Uniprot Description

ATP13A3: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily. 2 isoforms of the human protein are produced by alternative splicing

Protein type: EC 3.6.3.-; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: integral component of plasma membrane; intracellular membrane-bound organelle; membrane

Molecular Function: ATP binding; calcium-transporting ATPase activity; metal ion binding

Biological Process: cellular calcium ion homeostasis