Mouse anti-Human MTHFD2 Monoclonal Antibody | anti-MTHFD2 antibody

MTHFD2 (NMDMC, Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase, Mitochondrial, NAD-dependent Methylenetetrahydrofolate Dehydrogenase, Methenyltetrahydrofolate Cyclohydrolase) (PE)

Cat. Num. AAA6158929

• Gene Names: MTHFD2; NMDMC
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MTHFD2; Monoclonal Antibody; MTHFD2 (NMDMC; Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase; Mitochondrial; NAD-dependent Methylenetetrahydrofolate Dehydrogenase; Methenyltetrahydrofolate Cyclohydrolase) (PE); anti-MTHFD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4G7-2G3
• Specificity: Recognizes human MTHFD2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MTHFD2 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml detects MTHFD2 in human lymphoma tissue; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa16-344 from human MTHFD2 (AAH01548) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (62.3kD).)

Western Blot (WB)

(MTHFD2 monoclonal antibody Western Blot analysis of MTHFD2 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody. Lane 1: MTHFD2 transfected lysate (26.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml])

Product Categories/Family for anti-MTHFD2 antibody

Antibodies; Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 16306732
• NCBI GeneID: 10797
• Molecular Weight: 26,849 Da
• NCBI Official Full Name: Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase, mRNA
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• NCBI Official Symbol: MTHFD2
• NCBI Official Synonym Symbols: NMDMC
• NCBI Protein Information: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• Protein Family: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase

NCBI Description

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]