AXL recombinant protein

Recombinant AXL (470-894) protein

Cat. Num. AAA389380

• Gene Names: AXL; ARK; UFO; JTK11; Tyro7
• Synonyms: AXL; Recombinant AXL (470-894) protein; Tyrosine-protein kinase receptor UFO; AXL recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant AXL (470-894) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant AXL (470-894) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 48.9 kDa Purity: >85%)

Related Product Information for AXL recombinant protein

Short Description: Recombinant AXL (470-894) protein that includes amino acids 470-894 of human AXL protein (accession number NP_068713.2) with a N-terminal DYKDDDDK tag. The molecular weight of the protein is 48.9 kDa.

Background: AXL, also known as UFO, is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. AXL transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropinreleasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Product Categories/Family for AXL recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21536466
• NCBI GeneID: 558
• NCBI Accession #: NP_068713.2
• NCBI GenBank Nucleotide #: NM_021913.4
• UniProt Accession #: P30530; Q8N5L2; Q9UD27
• Molecular Weight: 97,377 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 1
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: ARK; UFO; JTK11; Tyro7
• NCBI Protein Information: tyrosine-protein kinase receptor UFO
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Protein kinase, TK; TK group; Axl family

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding

Biological Process: forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; organ regeneration; peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; vagina development; negative regulation of tumor necrosis factor production; signal transduction; phagocytosis; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; positive regulation of protein kinase B signaling cascade; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; blood vessel remodeling; spermatogenesis; negative regulation of neuron apoptosis; positive regulation of cytokine and chemokine mediated signaling pathway; inflammatory response; vascular endothelial growth factor receptor signaling pathway; apoptotic cell clearance; secretion by cell