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Western Blot (WB) (ATP13A3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ATP13A3 antibody detected the ATP13A3 protein (arrow).)

Rabbit anti-Human ATP13A3 Polyclonal Antibody | anti-ATP13A3 antibody

ATP13A3 Antibody (N-term)

Gene Names
ATP13A3; AFURS1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ATP13A3; Polyclonal Antibody; ATP13A3 Antibody (N-term); Probable cation-transporting ATPase 13A3; 363-; ATPase family homolog up-regulated in senescence cells 1; AFURS1; anti-ATP13A3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ATP13A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the N-terminal region of human ATP13A3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
101-129
Applicable Applications for anti-ATP13A3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Antigen Type
Synthetic Peptide
Clone Names
RB27285
Antigen Source
HUMAN
Cellular Location
Membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ATP13A3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ATP13A3 antibody detected the ATP13A3 protein (arrow).)

Western Blot (WB) (ATP13A3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ATP13A3 antibody detected the ATP13A3 protein (arrow).)

Immunohistochemistry (IHC)

(ATP13A3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP13A3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ATP13A3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP13A3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP13A3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ATP13A3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ATP13A3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
138043
NCBI Official Full Name
probable cation-transporting ATPase 13A3
NCBI Official Synonym Full Names
ATPase type 13A3
NCBI Official Symbol
ATP13A3
NCBI Official Synonym Symbols
AFURS1
NCBI Protein Information
probable cation-transporting ATPase 13A3
UniProt Protein Name
Probable cation-transporting ATPase 13A3
UniProt Gene Name
ATP13A3
UniProt Synonym Gene Names
AFURS1
UniProt Entry Name
AT133_HUMAN

NCBI Description

ATP13A3 is a member of the P-type ATPase family of proteins that transport a variety of cations across membranes. Other P-type ATPases include ATP7B (MIM 606882) and ATP7A (MIM 300011).[supplied by OMIM, Aug 2008]

Uniprot Description

ATP13A3: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Transporter, ion channel; Membrane protein, integral; Hydrolase; EC 3.6.3.-; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: membrane; integral to membrane

Molecular Function: metal ion binding; ATPase activity; ATP binding

Biological Process: metabolic process; cation transport

Research Articles on ATP13A3

Similar Products

Product Notes

The ATP13A3 atp13a3 (Catalog #AAA9204453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 101-129. The ATP13A3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP13A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the ATP13A3 atp13a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP13A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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