Rabbit ASRGL1 Polyclonal Antibody | anti-ASRGL1 antibody

ASRGL1 Antibody

Cat. Num. AAA9603580

• Gene Names: ASRGL1; ALP; ALP1; CRASH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ASRGL1; Polyclonal Antibody; ASRGL1 Antibody; ALP; ALP1; ASGL1; Asparaginase like 1; Asparaginase like 1 protein; Asparaginase like protein 1; ASRGL 1; beta-aspartyl-peptidase; CRASH; FLJ22316; isoaspartyl dipeptidase; isoaspartyl peptidase/L-asparaginase; L asparaginase; L asparagine amidohydrolase; anti-ASRGL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ASRGL1 antibody detects endogenous levels of ASRGL1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 308

• Applicable Applications for anti-ASRGL1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human ASRGL1.
• Subcellular Location: Cytoplasm. Note: Midpiece of sperm tail.
• Tissue Specificity: Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level). Over-expressed in uterine, mammary, prostatic and ovarian carcinoma.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (92%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (91%), Xenopus (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603580 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of HeLa cells, using ASRGL1 antibody.)

Related Product Information for anti-ASRGL1 antibody

Function: Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
Subunit Structure: Heterodimer of an alpha and beta chain produced by autocleavage. This heterodimer may then dimerize in turn, giving rise to a heterotetramer.
Post-translational Modifications: Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.
Similarity: Belongs to the Ntn-hydrolase family.

NCBI and Uniprot Product Information

• NCBI GI #: 145275202
• NCBI GeneID: 80150
• NCBI Accession #: NP_001077395.1
• NCBI GenBank Nucleotide #: NM_001083926.1
• UniProt Accession #: Q7L266; Q567Q4; Q6P1P0; Q8NI34; Q9H6F7; B2R7Q0
• Molecular Weight: Observed: 32 kDa; Predicted: 33 kDa
• NCBI Official Full Name: isoaspartyl peptidase/L-asparaginase
• NCBI Official Synonym Full Names: asparaginase like 1
• NCBI Official Symbol: ASRGL1
• NCBI Official Synonym Symbols: ALP; ALP1; CRASH
• NCBI Protein Information: isoaspartyl peptidase/L-asparaginase
• UniProt Protein Name: Isoaspartyl peptidase/L-asparaginase
• Protein Family: Isoaspartyl peptidase/L-asparaginase
• UniProt Gene Name: ASRGL1
• UniProt Synonym Gene Names: ALP; CRASH

Uniprot Description

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.