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Western Blot (WB) (Figure. Western Blot: Sample: Recombinant AST2, Mouse.)

Rabbit Aspartate Aminotransferase 2 (AST2) Polyclonal Antibody | anti-AST2 antibody

Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2)

Gene Names
Got2; Got-2; Kyat4; mAspAT; FABP-pm; AL022787
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Aspartate Aminotransferase 2 (AST2); Polyclonal Antibody; Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2); anti-AST2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.44mg/ml (varies by lot)
Applicable Applications for anti-AST2 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Rat
Immunogen
Recombinant AST2 (Ser30~Lys430) expressed in E.coli (MBS2010277)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot: Sample: Recombinant AST2, Mouse.)

Western Blot (WB) (Figure. Western Blot: Sample: Recombinant AST2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded kidney tissue))

Immunohistochemistry (IHC)

(HE staining on IHC-P; Samples: Mouse Liver Tissue.)

Immunohistochemistry (IHC) (HE staining on IHC-P; Samples: Mouse Liver Tissue.)

Immunohistochemistry (IHC)

(HE staining on IHC-P; Samples: Mouse Kidney Tissue.)

Immunohistochemistry (IHC) (HE staining on IHC-P; Samples: Mouse Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse AST2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ).)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse AST2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
aspartate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
glutamatic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol
Got2
NCBI Official Synonym Symbols
Got-2; Kyat4; mAspAT; FABP-pm; AL022787
NCBI Protein Information
aspartate aminotransferase, mitochondrial
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
Protein Family
UniProt Gene Name
Got2
UniProt Synonym Gene Names
Got-2; mAspAT; FABP-1; FABPpm

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.7; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 8 D1|8 47.79 cM

Cellular Component: cell surface; extracellular exosome; membrane; mitochondrial inner membrane; mitochondrion; myelin sheath; perikaryon; plasma membrane; protein complex

Molecular Function: amino acid binding; carboxylic acid binding; catalytic activity; enzyme binding; kynurenine-oxoglutarate transaminase activity; L-aspartate:2-oxoglutarate aminotransferase activity; phospholipid binding; protein homodimerization activity; pyridoxal phosphate binding; RNA binding; transaminase activity; transferase activity

Biological Process: amino acid metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; dicarboxylic acid metabolic process; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; lipid transport; oxaloacetate metabolic process; response to ethanol; transport

Research Articles on AST2

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Product Notes

The AST2 got2 (Catalog #AAA2001380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate Aminotransferase 2 (AST2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the AST2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase 2 (AST2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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