SIL1 recombinant protein

SIL1, 32-461aa, Human, His tag, E Coli

Cat. Num. AAA204276

• Gene Names: SIL1; BAP; MSS; ULG5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SIL1; 32-461aa; Human; His tag; E Coli; Nucleotide exchange factor SIL1; BAP; MSS; uLG5; SIL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MHQNLKEFAL TNPEKSSTKE TERKETKAEE ELDAEVLEVF HPTHEWQALQ PGQAVPAGSH VRLNLQTGER EAKLQYEDKF RNNLKGKRLD INTNTYTSQD LKSALAKFKE GAEMESSKED KARQAEVKRL FRPIEELKKD FDELNVVIET DMQIMVRLIN KFNSSSSSLE EKIAALFDLE YYVHQMDNAQ DLLSFGGLQV VINGLNSTEP LVKEYAAFVL GAAFSSNPKV QVEAIEGGAL QKLLVILATE QPLTAKKKVL FALCSLLRHF PYAQRQFLKL GGLQVLRTLV QEKGTEVLAV RVVTLLYDLV TEKMFAEEEA ELTQEMSPEK LQQYRQVHLL PGLWEQGWCE ITAHLLALPE HDAREKVLQT LGVLLTTCRD RYRQDPQLGR TLASLQAEYQ VLASLELQDG EDEGYFQELL GSVNSLLKEL RLEHHHHHH
• Sequence Length: 461

• Applicable Applications for SIL1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SIL1 recombinant protein

Nucleotide exchange factor, also known as SIL1, is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Recombinant human SIL1 protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SIL1 recombinant protein

Heat Shock Proteins

References

Chung KT., et al. (2002) J Biol Chem. 277 (49):47557-63.; Senderek J., et al. (2005) Nat Genet. 37 (12): 1312-4.

NCBI and Uniprot Product Information

• NCBI GI #: 11968009
• NCBI GeneID: 64374
• NCBI Accession #: NP_071909
• NCBI GenBank Nucleotide #: NM_022464.4
• UniProt Accession #: Q9H173; Q8N2L3; D3DQC2
• Molecular Weight: 50 kDa (439aa)
• NCBI Official Full Name: nucleotide exchange factor SIL1
• NCBI Official Synonym Full Names: SIL1 nucleotide exchange factor
• NCBI Official Symbol: SIL1
• NCBI Official Synonym Symbols: BAP; MSS; ULG5
• NCBI Protein Information: nucleotide exchange factor SIL1
• UniProt Protein Name: Nucleotide exchange factor SIL1
• Protein Family: Silaffin
• UniProt Gene Name: SIL1
• UniProt Synonym Gene Names: BAP

NCBI Description

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.