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Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A:293T; B: HeLa S3) using a 12% SDS PAGE gel and ARPC2 antibody at a dilution of 1:500)

Rabbit ARPC2 Polyclonal Antibody | anti-ARPC2 antibody

ARPC2 Antibody

Gene Names
ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
ARPC2; Polyclonal Antibody; ARPC2 Antibody; Affinity Purified Rabbit Polyclonal ARPC2 Antibody; Polyclonal ARPC2 antibody; Anti-ARPC2 antibody; actin-related protein 2/3 complex subunit 2 antibody; ARPC 2; PNAS-139 antibody; ARPC-2 antibody; ARPC2 antibody; ARPC-2; ARC34 antibody; p34-Arc antibody; ARPC 2 antibody; ARP2/3 protein complex subunit 34 antibody; anti-ARPC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
300
Applicable Applications for anti-ARPC2 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Immunogen
ARPC2 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 14 and 300 of ARPC2
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A:293T; B: HeLa S3) using a 12% SDS PAGE gel and ARPC2 antibody at a dilution of 1:500)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A:293T; B: HeLa S3) using a 12% SDS PAGE gel and ARPC2 antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 10% SDS PAGE gel and ARPC2 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 10% SDS PAGE gel and ARPC2 antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2 antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2 antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded OVCA xenograft using ARPC2 antibody at a dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded OVCA xenograft using ARPC2 antibody at a dilution of 1:100)
Related Product Information for anti-ARPC2 antibody
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined.
Product Categories/Family for anti-ARPC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34 kDa
NCBI Official Full Name
actin-related protein 2/3 complex subunit 2
NCBI Official Synonym Full Names
actin related protein 2/3 complex subunit 2
NCBI Official Symbol
ARPC2
NCBI Official Synonym Symbols
ARC34; PRO2446; p34-Arc; PNAS-139
NCBI Protein Information
actin-related protein 2/3 complex subunit 2
UniProt Protein Name
Actin-related protein 2/3 complex subunit 2
UniProt Gene Name
ARPC2
UniProt Synonym Gene Names
ARC34; p34-ARC
UniProt Entry Name
ARPC2_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ARPC2: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. Belongs to the ARPC2 family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; Arp2/3 protein complex; neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; synapse; cytosol; endosome; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; kinesin binding

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; cell motility

Research Articles on ARPC2

Similar Products

Product Notes

The ARPC2 arpc2 (Catalog #AAA5311839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ARPC2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the ARPC2 arpc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARPC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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