Rabbit anti-Human ARPC2 Polyclonal Antibody | anti-ARPC2 antibody

ARPC2, CT (ARPC2, ARC34, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34kD subunit) (Biotin)

Cat. Num. AAA6275338

• Gene Names: ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARPC2; Polyclonal Antibody; CT (ARPC2; ARC34; Actin-related protein 2/3 complex subunit 2; Arp2/3 complex 34kD subunit) (Biotin); Arp2/3 complex 34kD subunit; anti-ARPC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ARPC2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARPC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the C-terminal region of human ARPC2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARPC2 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ARPC2 antibody detected the ARPC2 protein (arrow).)

Related Product Information for anti-ARPC2 antibody

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq].

Product Categories/Family for anti-ARPC2 antibody

Antibodies; Actin Related Proteins

References

Monfregola, J., et al. J. Biol. Chem. 285(22):16951-16957(2010). Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010). Franke, A., et al. Nat. Genet. 40(11):1319-1323(2008). Xiao, F., et al. Brain Res. 1233, 168-175 (2008): Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 23238211
• NCBI GeneID: 10109
• NCBI Accession #: NP_690601.1
• NCBI GenBank Nucleotide #: NM_152862.2
• UniProt Accession #: O15144; Q92801; Q9P1D4
• Molecular Weight: 34,333 Da
• NCBI Official Full Name: actin-related protein 2/3 complex subunit 2
• NCBI Official Synonym Full Names: actin related protein 2/3 complex, subunit 2, 34kDa
• NCBI Official Symbol: ARPC2
• NCBI Official Synonym Symbols: ARC34; PRO2446; p34-Arc; PNAS-139
• NCBI Protein Information: actin-related protein 2/3 complex subunit 2; arp2/3 complex 34 kDa subunit; ARP2/3 protein complex subunit 34
• UniProt Protein Name: Actin-related protein 2/3 complex subunit 2
• UniProt Gene Name: ARPC2
• UniProt Synonym Gene Names: ARC34; p34-ARC
• UniProt Entry Name: ARPC2_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ARPC2: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. Belongs to the ARPC2 family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; Arp2/3 protein complex; neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; synapse; cytosol; endosome; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; kinesin binding

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; cell motility