Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).hARNT immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human ARNT Polyclonal Antibody | anti-ARNT antibody
ARNT, ID (V528) (ARNT, BHLHE2, Aryl hydrocarbon receptor nuclear translocator, Class E basic helix-loop-helix protein 2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta)
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).hARNT immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).hARNT immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).)
Western Blot (WB)
(ARNT Antibody (V528) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]
Uniprot Description
ARNT: a bHLH transcription factor that requires dimerization with another bHLH protein for efficient DNA binding. A binding partner for the Ah (dioxin) nuclear receptor. Required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. A binding partner for HIF1A or HIF2A, transcription factors that regulates transcription from RNA polymerase II promoter in the adaptive response to hypoxia and oxidative stress. This complex activates target genes that limit oxygen consumption. HIF-1alpha may have nontranscriptional activities that inhibit DNA replication and cell proliferation when oxygen becomes scarce. Forms heterodimers with other bHLH proteins. Interacts with TACC3. Three isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor; Transcription factor; Motility/polarity/chemotaxis; DNA-binding
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; aryl hydrocarbon receptor binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; aryl hydrocarbon receptor activity; transcription factor binding; transcription factor activity
Biological Process: embryonic placenta development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; mRNA transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein sumoylation; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; cell differentiation; regulation of transcription from RNA polymerase II promoter in response to oxidative stress
Research Articles on ARNT
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Product Notes
The ARNT arnt (Catalog #AAA6003275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARNT, ID (V528) (ARNT, BHLHE2, Aryl hydrocarbon receptor nuclear translocator, Class E basic helix-loop-helix protein 2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARNT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100. Researchers should empirically determine the suitability of the ARNT arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARNT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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