Human MutY Homolog (MUTYH) ELISA Kit | MUTYH elisa kit

Human MutY Homolog (MUTYH) ELISA Kit

Cat. Num. AAA451468

• Gene Names: MUTYH; MYH
• Reactivity: Human
• Synonyms: MutY Homolog (MUTYH); Human MutY Homolog (MUTYH) ELISA Kit; MYH; A/G-specific adenine DNA glycosylase; MUTYH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MUTYH. No significant cross-reactivity or interference between MUTYH and analogues was observed.
• Sequence Length: 535

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.041ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUTYH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUTYH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUTYH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUTYH in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUTYH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUTYH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUTYH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUTYH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115298648
• NCBI GeneID: 4595
• NCBI Accession #: NP_001041636.1
• NCBI GenBank Nucleotide #: NM_001048171.1
• UniProt Accession #: Q9UIF7; Q15830; Q9UBP2; Q9UBS7; Q9UIF4; Q9UIF5; Q9UIF6; D3DPZ4
• Molecular Weight: 57,446 Da
• NCBI Official Full Name: adenine DNA glycosylase isoform 2
• NCBI Official Synonym Full Names: mutY DNA glycosylase
• NCBI Official Symbol: MUTYH
• NCBI Official Synonym Symbols: MYH
• NCBI Protein Information: adenine DNA glycosylase
• UniProt Protein Name: Adenine DNA glycosylase
• UniProt Gene Name: MUTYH
• UniProt Synonym Gene Names: MYH; hMYH

NCBI Description

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.