Immunohistochemistry (IHC)
(Anti-ARIH1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Goat ARIH1 Polyclonal Antibody | anti-ARIH1 antibody
Goat Polyclonal to Human ARIH1
Gene Names
ARIH1; ARI; HARI; HHARI; UBCH7BP
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
ARIH1; Polyclonal Antibody; Goat Polyclonal to Human ARIH1; Anti-ARIH1 Antibody (C-Terminus) IHC-plus; ARI; HARI; Monocyte protein 6; MOP-6; Protein ariadne-1 homolog; UbcH7-binding protein; ARI-1; UbcM4-interacting protein; H7-AP2; HHARI; MOP6; UBCH7BP; Human ARIH1; anti-ARIH1 antibody
Host
Goat
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Zebrafish
Clonality
Polyclonal
Specificity
Human ARIH1.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
557
Applicable Applications for anti-ARIH1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.2 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic peptide C-HEGYEKDLWEYIED from the C-terminus of human ARIH1 (NP_005735.2).
Immunogen Type
Synthetic peptide
Immunogen
ARIH1 antibody was raised against synthetic peptide C-HEGYEKDLWEYIED from the C-terminus of human ARIH1 (NP_005735.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (100%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Anti-ARIH1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-ARIH1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Antibody staining (0.5 ug/ml) of U937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)
Western Blot (WB)
(Antibody staining (0.5 ug/ml) of U937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,118 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase ARIH1
NCBI Official Synonym Full Names
ariadne RBR E3 ubiquitin protein ligase 1
NCBI Official Symbol
ARIH1
NCBI Official Synonym Symbols
ARI; HARI; HHARI; UBCH7BP
NCBI Protein Information
E3 ubiquitin-protein ligase ARIH1; ARI-1; MOP-6; H7-AP2; monocyte protein 6; ubcH7-binding protein; protein ariadne-1 homolog; ubcM4-interacting protein; ariadne, Drosophila, homolog of; ubiquitin-conjugating enzyme E2-binding protein 1; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1
UniProt Protein Name
E3 ubiquitin-protein ligase ARIH1
Protein Family
UniProt Gene Name
ARIH1
UniProt Synonym Gene Names
ARI; MOP6; UBCH7BP; MOP-6; ARI-1
UniProt Entry Name
ARI1_HUMAN
Uniprot Description
ARIH1: E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Belongs to the RBR family. Ariadne subfamily.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: cytoplasm; cytosol; ubiquitin ligase complex
Molecular Function: protein binding; small conjugating protein ligase activity; zinc ion binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; protein ubiquitination
Research Articles on ARIH1
Similar Products
Product Notes
The ARIH1 arih1 (Catalog #AAA242134) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ARIH1 reacts with Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ARIH1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:32000), IHC-P (5 ug/ml), WB (0.2 - 1 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the ARIH1 arih1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARIH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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