Western Blot (WB)
(Anti-ARIH1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:64 kDa)
Rabbit ARIH1 Polyclonal Antibody | anti-ARIH1 antibody
Anti-ARIH1 Antibody, Rabbit Polyclonal
Gene Names
ARIH1; ARI; HARI; HHARI; UBCH7BP
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ARIH1; Polyclonal Antibody; Anti-ARIH1 Antibody; Rabbit Polyclonal; ARIH1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ARI Antibody; Anti-HARI Antibody; Anti-HHARI Antibody; Anti-HUSSY-27 Antibody; Anti-UBCH7BP Antibody; anti-ARIH1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ARIH1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ARIH1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human ARIH1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ARIH1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ARIH1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:64 kDa)
Western Blot (WB)
(Anti-ARIH1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:64 kDa)
Related Product Information for anti-ARIH1 antibody
The specificity of the ubiquitin degradation system is regulated through interaction between individual ubiquitin-conjugating enzymes (E2s) and multiple ubiquitin-protein ligases (E3s). ARIH1/HHARI is an E3 ubiquitin ligase that triggers mitophagy in cancer cells in a PINK1-dependent manner. ARIH1 is widely expressed in cancer cells, notably in breast and lung adenocarcinomas. ARIH1 expression protects against chemotherapy-induced death. ARIH1 enhances cisplatin resistance in normal and melanoma cells. By regulating ARIH1, neurofibromin 2, a tumor suppressor, enhances cisplatin resistance in melanoma but not normal cells.
References
Villa E, et al. (2017) Parkin-independent mitophagy controls chemotherapeutic response in cancer cells. Cell Rep 20 (12): 2846-2859.Tan NG, et al. (2000) Characterisation of the human and mouse orthologues of the drosophila ariadne gene. Cytogenet Cell Genet 90 (3-4): 242-245.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,118 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase ARIH1
NCBI Official Synonym Full Names
ariadne RBR E3 ubiquitin protein ligase 1
NCBI Official Symbol
ARIH1
NCBI Official Synonym Symbols
ARI; HARI; HHARI; UBCH7BP
NCBI Protein Information
E3 ubiquitin-protein ligase ARIH1; ARI-1; MOP-6; H7-AP2; monocyte protein 6; ubcH7-binding protein; protein ariadne-1 homolog; ubcM4-interacting protein; ariadne, Drosophila, homolog of; ubiquitin-conjugating enzyme E2-binding protein 1; ariadne homolog
UniProt Protein Name
E3 ubiquitin-protein ligase ARIH1
Protein Family
UniProt Gene Name
ARIH1
UniProt Synonym Gene Names
ARI; MOP6; UBCH7BP; MOP-6; ARI-1
UniProt Entry Name
ARI1_HUMAN
Uniprot Description
ARIH1: E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Belongs to the RBR family. Ariadne subfamily.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: cytoplasm; cytosol; ubiquitin ligase complex
Molecular Function: protein binding; small conjugating protein ligase activity; zinc ion binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; protein ubiquitination
Research Articles on ARIH1
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Product Notes
The ARIH1 arih1 (Catalog #AAA8102397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARIH1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's ARIH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ARIH1 arih1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARIH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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