Western Blot (WB)
((0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Desmin Polyclonal Antibody | anti-DES antibody
Goat anti-Desmin Antibody
Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm and cell membrane staining in stromal cells. Recommended concentration, 3-6ug/ml. The data were obtained from a previous batch (different goat).
Western Blot: Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to NP_001918.3). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Uniprot Description
desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: cytosol; fascia adherens; intermediate filament; intermediate filament cytoskeleton; neuromuscular junction; sarcolemma; Z disc
Molecular Function: cytoskeletal protein binding; identical protein binding; protein binding; structural constituent of cytoskeleton
Biological Process: cytoskeleton organization and biogenesis; intermediate filament organization; muscle contraction; muscle filament sliding; regulation of heart contraction
Disease: Cardiomyopathy, Dilated, 1i; Muscular Dystrophy, Limb-girdle, Type 2r; Myopathy, Myofibrillar, 1; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type
Research Articles on DES
Similar Products
Product Notes
The DES des (Catalog #AAA422190) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Desmin Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Desmin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm and cell membrane staining in stromal cells. Recommended concentration, 3-6ug/ml. The data were obtained from a previous batch (different goat). Western Blot: Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to NP_001918.3). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the DES des for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDGEVVSEAT QQQHE. It is sometimes possible for the material contained within the vial of "Desmin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
