Rabbit ARHGDIA Polyclonal Antibody | anti-ARHGDIA antibody

ARHGDIA (Phospho-Ser174) Antibody

Cat. Num. AAA9502916

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: ARHGDIA; Polyclonal Antibody; ARHGDIA (Phospho-Ser174) Antibody; GDIR; Rho GDI 1; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; anti-ARHGDIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: ARHGDIA (Phospho-Ser174) Antibody detects endogenous levels of ARHGDIA only when phosphorylated at Ser174.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-ARHGDIA antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S174; M:S174; R:S174
• Immunogen: The antiserum was produced against synthesized peptide derived from human ARHGDIA around the phosphorylation site of Ser174.
• Immunogen Range: 140-189
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 297374782
• NCBI GeneID: 396
• NCBI Accession #: NP_001172006.1
• NCBI GenBank Nucleotide #: NM_001185077.2
• UniProt Accession #: P52565; Q6IBM5; A8MXW0; B2R5X1; B4DDD3; B4DUV9
• Molecular Weight: 23 kDa
• NCBI Official Full Name: rho GDP-dissociation inhibitor 1 isoform a
• UniProt Protein Name: Rho GDP-dissociation inhibitor 1
• UniProt Gene Name: ARHGDIA
• UniProt Synonym Gene Names: GDIA1; Rho GDI 1

Uniprot Description

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.