Rabbit ARHGDIA Polyclonal Antibody | anti-ARHGDIA antibody

ARHGDIA Antibody

Cat. Num. AAA9504023

• Gene Names: ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: ARHGDIA; Polyclonal Antibody; ARHGDIA Antibody; GDIR; Rho GDI 1; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; anti-ARHGDIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: ARHGDIA Antibody detects endogenous levels of total ARHGDIA protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-ARHGDIA antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:5000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human ARHGDIA.
• Immunogen Range: 140-189
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using ARHGDIA Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 297374782
• NCBI GeneID: 396
• NCBI Accession #: NP_001172006.1
• NCBI GenBank Nucleotide #: NM_001185077.2
• UniProt Accession #: P52565; Q6IBM5; A8MXW0; B2R5X1; B4DDD3; B4DUV9
• Molecular Weight: 23 kDa
• NCBI Official Full Name: rho GDP-dissociation inhibitor 1 isoform a
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor alpha
• NCBI Official Symbol: ARHGDIA
• NCBI Official Synonym Symbols: GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
• NCBI Protein Information: rho GDP-dissociation inhibitor 1
• UniProt Protein Name: Rho GDP-dissociation inhibitor 1
• UniProt Gene Name: ARHGDIA
• UniProt Synonym Gene Names: GDIA1; Rho GDI 1

NCBI Description

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.