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Western Blot (WB) (Western Blot analysis of ARHGAP25 in Hela cells using ARHGAP25 Rabbit pAb at dilution 1/250)

Rabbit anti-Human ARHGAP25 Polyclonal Antibody | anti-ARHGAP25 antibody

ARHGAP25 Rabbit pAb

Gene Names
ARHGAP25; KAIA0053; HEL-S-308
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
ARHGAP25; Polyclonal Antibody; ARHGAP25 Rabbit pAb; Rho GTPase activating protein 25; KAIA0053; anti-ARHGAP25 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-ARHGAP25 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:50-1:200
Immunogen
Fusion protein of human ARHGAP25
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of ARHGAP25 in Hela cells using ARHGAP25 Rabbit pAb at dilution 1/250)

Western Blot (WB) (Western Blot analysis of ARHGAP25 in Hela cells using ARHGAP25 Rabbit pAb at dilution 1/250)

Immunohistochemistry (IHC)

(Immunohistochemistry of ARHGAP25 in paraffin-embedded Human thyroid cancer tissue using ARHGAP25 Rabbit pAb at dilution 1/40. Predicted cell location: Cytoplasm or Nucleus)

Immunohistochemistry (IHC) (Immunohistochemistry of ARHGAP25 in paraffin-embedded Human thyroid cancer tissue using ARHGAP25 Rabbit pAb at dilution 1/40. Predicted cell location: Cytoplasm or Nucleus)
Related Product Information for anti-ARHGAP25 antibody
Rho GTPase activating protein 25 is a protein that in humans is encoded by the ARHGAP25 gene. The gene is also known as KAIA0053. ARHGAP25 belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling, cell polarity, and cell migration. GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
645
NCBI Official Full Name
Rho GTPase-activating protein 25
NCBI Official Synonym Full Names
Rho GTPase activating protein 25
NCBI Official Symbol
ARHGAP25
NCBI Official Synonym Symbols
KAIA0053; HEL-S-308
NCBI Protein Information
rho GTPase-activating protein 25; epididymis secretory protein Li 308; rho-type GTPase-activating protein 25
UniProt Protein Name
Rho GTPase-activating protein 25
UniProt Gene Name
ARHGAP25
UniProt Synonym Gene Names
KIAA0053
UniProt Entry Name
RHG25_HUMAN

NCBI Description

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]

Uniprot Description

ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity

Research Articles on ARHGAP25

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Product Notes

The ARHGAP25 arhgap25 (Catalog #AAA4759999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP25 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the ARHGAP25 arhgap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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