Rabbit anti-Human AKR1A1 Polyclonal Antibody | anti-AKR1A1 antibody

AKR1A1, NT (Alcohol Dehydrogenase [NADP+], Aldehyde Reductase, Aldo-keto Reductase Family 1 Member A1, ALDR1, ALR) (HRP)

Cat. Num. AAA6462428

• Gene Names: AKR1A1; ALR; ARM; DD3; ALDR1; HEL-S-6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: AKR1A1; Polyclonal Antibody; NT (Alcohol Dehydrogenase [NADP+]; Aldehyde Reductase; Aldo-keto Reductase Family 1 Member A1; ALDR1; ALR) (HRP); anti-AKR1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AKR1A1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AKR1A1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human AKR1A1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-AKR1A1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.)

Product Categories/Family for anti-AKR1A1 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Steuber, H., J. Mol. Biol. 379(5), 991-1016 (2008). 2. Bohren, K.M., Biochim. Biophys. Acta 1748(2), 201-212 (2005). 3. El-Kabbani, O., Acta Crystallogr. D Biol. Crystallogr. 50(PT 6), 859-868 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 5174391
• NCBI GeneID: 10327
• NCBI Accession #: NP_006057
• NCBI GenBank Nucleotide #: NM_006066.3
• UniProt Accession #: Q6IAZ4; A8KAL8; D3DQ04; P14550
• Molecular Weight: 36.5 kDa (325aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: alcohol dehydrogenase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member A1
• NCBI Official Symbol: AKR1A1
• NCBI Official Synonym Symbols: ALR; ARM; DD3; ALDR1; HEL-S-6
• NCBI Protein Information: alcohol dehydrogenase [NADP(+)]
• Protein Family: Alcohol dehydrogenase

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]