Rabbit anti-Human ARHGAP22 Polyclonal Antibody | anti-ARHGAP22 antibody

ARHGAP22, CT (ARHGAP22, RHOGAP2, Rho GTPase-activating protein 22, Rho-type GTPase-activating protein 22) (Azide free) (HRP)

Cat. Num. AAA6274867

• Gene Names: ARHGAP22; RhoGAP2; RhoGap22
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARHGAP22; Polyclonal Antibody; CT (ARHGAP22; RHOGAP2; Rho GTPase-activating protein 22; Rho-type GTPase-activating protein 22) (Azide free) (HRP); Rho-type GTPase-activating protein 22; anti-ARHGAP22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 698

• Applicable Applications for anti-ARHGAP22 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARHGAP22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-633 amino acids from the C-terminal region of human ARHGAP22.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARHGAP22 Antibody (C-term) western blot analysis in MDA-MB231,WiDr,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ARHGAP22 antibody detected the ARHGAP22 protein (arrow).)

Related Product Information for anti-ARHGAP22 antibody

ARHGAPs, such as ARHGAP22, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).

Product Categories/Family for anti-ARHGAP22 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Dick, D.M., et al. Mol. Psychiatry (2010). In press: Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Katoh, M., et al. Int. J. Mol. Med. 14(2):333-338(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 34013590
• NCBI GeneID: 58504
• NCBI Accession #: NP_067049.2
• NCBI GenBank Nucleotide #: NP_067049.2
• UniProt Accession #: Q7Z5H3
• NCBI Official Full Name: rho GTPase-activating protein 22 isoform 3
• NCBI Official Synonym Full Names: Rho GTPase activating protein 22
• NCBI Official Symbol: ARHGAP22
• NCBI Official Synonym Symbols: RhoGAP2; RhoGap22
• NCBI Protein Information: rho GTPase-activating protein 22
• UniProt Protein Name: Rho GTPase-activating protein 22
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP22
• UniProt Synonym Gene Names: RHOGAP2
• UniProt Entry Name: RHG22_HUMAN

NCBI Description

This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ARHGAP22: Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: focal adhesion; nucleus; cytosol

Molecular Function: protein binding; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; angiogenesis; cell differentiation; positive regulation of GTPase activity