Rabbit anti-Human HSF1 Polyclonal Antibody | anti-HSF1 antibody

HSF1, NT (HSF1, HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1) (APC)

Cat. Num. AAA6308541

• Gene Names: HSF1; HSTF1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HSF1; Polyclonal Antibody; NT (HSF1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1) (APC); Heat shock transcription factor 1; anti-HSF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HSF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the N-terminal region of human HSF1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.)

Immunohistochemistry (IHC)

(HSF1 Antibody (N-term) (AP14189a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HSF1 antibody

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.

Product Categories/Family for anti-HSF1 antibody

Antibodies; Transcription Factors

References

Hayashida, N., et al. EMBO J. 29(20):3459-3469(2010). Sangle, G.V., et al. Endocrinology 151(9):4455-4466(2010). Shunmei, E., et al. Mol. Cells 29(5):527-531(2010). Kim, S.A., et al. Int. J. Oncol. 36(4):867-872(2010). Wang, C., et al. J Transl Med 8, 44 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5031767
• NCBI GeneID: 3297
• NCBI Accession #: NP_005517.1
• NCBI GenBank Nucleotide #: NM_005526.2
• UniProt Accession #: Q00613; Q53XT4; A8K4L0; A8MW26
• Molecular Weight: 57,260 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock transcription factor 1
• NCBI Official Symbol: HSF1
• NCBI Official Synonym Symbols: HSTF1
• NCBI Protein Information: heat shock factor protein 1; HSF 1; HSTF 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSTF1; HSF 1; HSTF 1
• UniProt Entry Name: HSF1_HUMAN

NCBI Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: negative regulation of cell proliferation; embryonic placenta development; embryonic process involved in female pregnancy; mRNA transcription; female meiosis; defense response; spermatogenesis; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation