Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using ARHGAP10 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ARHGAP10 Polyclonal Antibody | anti-ARHGAP10 antibody
ARHGAP10 Antibody
Gene Names
ARHGAP21; ARHGAP10
Reactivity
Human, MousePredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ARHGAP10; Polyclonal Antibody; ARHGAP10 Antibody; Arhgap21; KIAA1424; RHG21_HUMAN; Rho GTPase-activating protein 10; Rho GTPase-activating protein 21; Rho-type GTPase-activating protein 21; anti-ARHGAP10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
ARHGAP10 Antibody detects endogenous levels of ARHGAP10.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1958
Applicable Applications for anti-ARHGAP10 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human ARHGAP10.
Tissue Specificity
Widely expressed with higher expression in brain, heart, skeletal muscle and placenta.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Cytoskeleton; Golgi Apparatus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using ARHGAP10 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using ARHGAP10 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using ARHGAP10 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Myeloma cells, using ARHGAP10 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ARHGAP10 antibody
Description: Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions.
Post Translational Modifications: Sumoylated with SUMO2 and SUMO3 in proliferating lymphocytes.
Subunit Structure: Interacts with GTP-bound ARF1 and ARF6. Interacts with CTNNA1.
Post Translational Modifications: Sumoylated with SUMO2 and SUMO3 in proliferating lymphocytes.
Subunit Structure: Interacts with GTP-bound ARF1 and ARF6. Interacts with CTNNA1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 89 kDa
Predicted: 218 kDa
Predicted: 218 kDa
NCBI Official Full Name
rho GTPase-activating protein 21 isoform a
NCBI Official Synonym Full Names
Rho GTPase activating protein 21
NCBI Official Symbol
ARHGAP21
NCBI Official Synonym Symbols
ARHGAP10
NCBI Protein Information
rho GTPase-activating protein 21
UniProt Protein Name
Rho GTPase-activating protein 21
Protein Family
UniProt Gene Name
ARHGAP21
UniProt Synonym Gene Names
ARHGAP10; KIAA1424
UniProt Entry Name
RHG21_HUMAN
NCBI Description
ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008]
Research Articles on ARHGAP10
Similar Products
Product Notes
The ARHGAP10 arhgap21 (Catalog #AAA9611581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP10 Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ARHGAP10 arhgap21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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