Western Blot (WB)
(Western blot analysis of extracts from Mouse liver tissue?, using CBY1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit CBY1 Polyclonal Antibody | anti-CBY1 antibody
CBY1 Antibody
Gene Names
CBY1; CBY; arb1; PGEA1; C22orf2; Chibby1; PIGEA14; PIGEA-14; HS508I15A
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
CBY1; Polyclonal Antibody; CBY1 Antibody; ARPP-binding protein; C22orf2; CBY; Cby1; CBY1_HUMAN; Cytosolic leucine rich protein; Cytosolic leucine-rich protein; Golgi and endoplasmic reticulum-associated 1; HRIHFB2025; PIGEA 14; PIGEA-14; PKD2 interactor; Protein Chibby; Protein chibby homolog 1; anti-CBY1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
CBY1 Antibody detects endogenous levels of CBY1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
169
Applicable Applications for anti-CBY1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human CBY1.
Tissue Specificity
Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Cytoskeleton; Golgi Apparatus; Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse liver tissue?, using CBY1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse liver tissue?, using CBY1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CBY1 antibody
Description: Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
Subunit Structure: Homodimer. Interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of CTNNB1 (PubMed:12712206, PubMed:16424001). Interacts (C-terminus) with TCIM (C-terminus), TCIM competes with CTNNB1 for the interaction with CBY1 (PubMed:16424001). Interacts with FAM92A; this interaction facilites targeting of FAM92A to cilium basal body (PubMed:27528616).
Similarity: Belongs to the chibby family.
Subunit Structure: Homodimer. Interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of CTNNB1 (PubMed:12712206, PubMed:16424001). Interacts (C-terminus) with TCIM (C-terminus), TCIM competes with CTNNB1 for the interaction with CBY1 (PubMed:16424001). Interacts with FAM92A; this interaction facilites targeting of FAM92A to cilium basal body (PubMed:27528616).
Similarity: Belongs to the chibby family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 30 kDa
Predicted: 15 kDa
Predicted: 15 kDa
NCBI Official Full Name
protein chibby homolog 1 isoform b
NCBI Official Synonym Full Names
chibby family member 1, beta catenin antagonist
NCBI Official Symbol
CBY1
NCBI Official Synonym Symbols
CBY; arb1; PGEA1; C22orf2; Chibby1; PIGEA14; PIGEA-14; HS508I15A
NCBI Protein Information
protein chibby homolog 1
UniProt Protein Name
Protein chibby homolog 1
Protein Family
UniProt Gene Name
CBY1
UniProt Synonym Gene Names
ARB1; C22orf2; CBY; PGEA1; HRIHFB2025
UniProt Entry Name
CBY1_HUMAN
NCBI Description
Beta-catenin is a transcriptional activator and oncoprotein involved in the development of several cancers. The protein encoded by this gene interacts directly with the C-terminal region of beta-catenin, inhibiting oncogenic beta-catenin-mediated transcriptional activation by competing with transcription factors for binding to beta-catenin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on CBY1
Similar Products
Product Notes
The CBY1 cby1 (Catalog #AAA9611633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CBY1 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CBY1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CBY1 cby1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBY1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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