Rabbit Ser316 NF-kappaB p65 Polyclonal Antibody | anti-RELA antibody

Anti-Phospho-Ser316 NF-kappaB p65 Antibody

Cat. Num. AAA500087

• Gene Names: Rela; p65; p65 NFkB; p65 NF-kappa B
• Reactivity: Human, Mouse; Predicted: Bovine, Canine, Goat, Hamster, Pig, Rat
• Applications: Western Blot
• Purity: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Synonyms: Ser316 NF-kappaB p65; Polyclonal Antibody; Anti-Phospho-Ser316 NF-kappaB p65 Antibody; NF-kB p65 (Ser316); anti-RELA antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine, Canine, Goat, Hamster, Pig, Rat
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the~65 kDa NF-kappaB p65 protein phosphorylated at Ser316. Immunolabeling is nearly eliminated with Lambda-Ptase treatment.
• Purity/Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 549

• Applicable Applications for anti-RELA antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Mouse
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser316 conjugated to KLH.
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 316 of mouse NF-kappaB p65.
• Quality Control Tests: Western blots performed on each lot.
• Preparation and Storage: Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.

Western Blot (WB)

(Western blot of 20' UV treated HeLa lysate showing specific immunolabeling of the ~65 kDa NF-?B p65 phosphorylated at Ser 316 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)

Related Product Information for anti-RELA antibody

p65, also known as RelA, is one of the five members of the NF-kappaB family. The p65 and p50 heterodimer is the predominant form of NF-kappaB, where p65 is the major subunit (Gilmore et al, 2006). There have been 13 identified phosphorylation sites on p65, including ser316 which is induced by IL-1beta (Viatour et al, 2005 and Wang et al, 2015). Phosphorylation of ser316 is essential for NF-kappaB nuclear transportation, NF-kappaB-dependent gene regulation, cytokine/chemokine secretion, and other biological functions (Wang et al, 2015). Ser316 has also been demonstrated to cophosphorylate with either Ser529 or Ser536 to regulate the majority of NF-kappaB targeted genes while having its own unique regulation function (Wang et al, 2015). CKI kinase specifically targets phosphorylation at ser316 on p65 (Wang et al, 2015). CKI is involved in regulation of membrane transport, cell division, DNA repair, and nuclear localization by phosphorylating its target proteins (Price et al, 2006).

References

1. Gilmore, TD (2006) Introduction to NF-kappaB: players, pathways, perspecitives. Oncogene 25, 6680-6684. 2. Viatour P, Merville, MP, Bours V, Chariot A. (2005) Phosphorylation of NF- kappaB and IkappaB proteins: implications in cancer and inflammation. Trends Biochem. Sci. 30, 43-52. 3. Wang B, Wei H, Prabhu L, Zhao W, Martin M, Hartley AV, and Lu T. (2015) Role of Novel Serine 316 Phosphorylation of the p65 Subunit of NF-kappaB in Differential Gene Regulation. J Biol Chem, 290(33):20336-47. 4. Price, MA. (2006) CKI, there's more than one: casein kinase I family members in Wnt and Hedgehog signaling. Gene Dev. 20, 399-410.

NCBI and Uniprot Product Information

• NCBI GI #: 6677709
• NCBI GeneID: 19697
• NCBI Accession #: NP_033071.1
• NCBI GenBank Nucleotide #: NM_009045.5
• UniProt Accession #: Q04207
• Molecular Weight: ~65 kDa
• NCBI Official Full Name: transcription factor p65 isoform 1
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65; p65 NFkB; p65 NF-kappa B
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Cellular Component: cytoplasm; cytosol; nucleus; protein complex; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; enzyme binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cellular response to stress; cytokine and chemokine mediated signaling pathway; defense response; hair follicle development; inflammatory response; innate immune response; liver development; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; regulation of transcription, DNA-dependent; response to bacterium; response to cytokine stimulus; response to muramyl dipeptide; response to organic substance; response to UV-B; transcription from RNA polymerase II promoter; transcription, DNA-dependent