Western Blot (WB)
(ARGFX Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARGFX antibody detected the ARGFX protein (arrow).)
Rabbit anti-Human ARGFX Polyclonal Antibody | anti-ARGFX antibody
ARGFX, ID (ARGFX, Arginine-fifty homeobox) (APC)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(ARGFX Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARGFX antibody detected the ARGFX protein (arrow).)
Western Blot (WB)
(ARGFX Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARGFX antibody detected the ARGFX protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody (Center).HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody (Center).HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.)
NCBI and Uniprot Product Information
NCBI Description
Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the ARGFX homeobox gene family. [provided by RefSeq, Jul 2008]
Uniprot Description
ARGFX: Putative transcription factor. Belongs to the paired homeobox family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Product Notes
The ARGFX argfx (Catalog #AAA6274798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARGFX, ID (ARGFX, Arginine-fifty homeobox) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARGFX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARGFX argfx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARGFX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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