ELISA (EIA)
(To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Cat. #208604 (biotinylated version of B2553-07C) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2.)
Rabbit anti-Human Bone Morphogenetic Protein 2 Polyclonal Antibody | anti-BMP2 antibody
Bone Morphogenetic Protein 2 (BMP2) (AP)
Reactivity
Human
Applications
Neutralization Assay, ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Bone Morphogenetic Protein 2; Polyclonal Antibody; Bone Morphogenetic Protein 2 (BMP2) (AP); anti-BMP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Bone Morphogenetic Protein 2 (BMP-2).
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-BMP2 antibody
Neutralization, ELISA (EIA), Western Blot (WB)
Application Notes
Neutralization: 1.3-1.9ug/ml. Yields one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200ng/ml).
ELISA: 0.5-2ug/ml (100ul/well); Detects 0.2-0.4ng/well of recombinant hBMP-2.
WB: 0.1-0.2ug/ml. Detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
ELISA: 0.5-2ug/ml (100ul/well); Detects 0.2-0.4ng/well of recombinant hBMP-2.
WB: 0.1-0.2ug/ml. Detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
Immunogen
Highly pure (>98%) recombinant human BMP-2.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
ELISA (EIA)
(To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Cat. #208604 (biotinylated version of B2553-07C) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2.)
ELISA (EIA)
(To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Cat. #208604 (biotinylated version of B2553-07C) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2.)
Product Categories/Family for anti-BMP2 antibody
Similar Products
Product Notes
The BMP2 (Catalog #AAA6465494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bone Morphogenetic Protein 2 (BMP2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bone Morphogenetic Protein 2 can be used in a range of immunoassay formats including, but not limited to, Neutralization, ELISA (EIA), Western Blot (WB). Neutralization: 1.3-1.9ug/ml. Yields one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200ng/ml). ELISA: 0.5-2ug/ml (100ul/well); Detects 0.2-0.4ng/well of recombinant hBMP-2. WB: 0.1-0.2ug/ml. Detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BMP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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