Western Blot (WB)
(Western Blot (WB) analysis of NIP7 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse kidney tissue lysateLane3:H9C2 whole cell lysate)
Rabbit NIP7 Polyclonal Antibody | anti-NIP7 antibody
NIP7 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NIP7; Polyclonal Antibody; NIP7 Polyclonal Antibody; 60S ribosome subunit biogenesis protein NIP7 homolog; KD93; NIP-7; CGI-37; HSPC031; HSPC180; OK/SW-cl.76; OK/SW-cl.78; anti-NIP7 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NIP7 polyclonal antibody detects endogenous levels of NIP7 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
133
Applicable Applications for anti-NIP7 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human NIP7.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NIP7 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse kidney tissue lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of NIP7 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse kidney tissue lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-NIP7 antibody
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Nip7 is a nucleolar protein involved in ribosome biogenesis, specifically 27S pre-rRNA processing and 60S ribosome subunit assembly in Saccharomyces cerevisiae. NIP7 is a conserved protein among eukaryotes, including human, mouse, rat and pig that is essential for cell growth. In humans, NIP7 interacts with the Shwachman-Bodian-Diamond syndrome (SBDS) protein, which mediates accurate gene expression essential for proper brain, skeletal, and blood cell development. Mutations in the SBDS gene results in an autosomal disorder (SDS) characterized by pleiotropic phenotypes including pancreatic, skeletal and bone marrow deficiencies and predisposition to hematological dysfunctions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 20kDa
NCBI Official Full Name
60S ribosome subunit biogenesis protein NIP7 homolog isoform 2
UniProt Protein Name
60S ribosome subunit biogenesis protein NIP7 homolog
Protein Family
UniProt Gene Name
NIP7
UniProt Entry Name
NIP7_HUMAN
Uniprot Description
NIP7: Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly. Belongs to the NIP7 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Nucleolus
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: nucleolus
Molecular Function: protein binding
Biological Process: ribosome assembly
Similar Products
Product Notes
The NIP7 nip7 (Catalog #AAA3006369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NIP7 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NIP7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the NIP7 nip7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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