Western Blot (WB)
(Western blot analysis of MBS615326 in Daudi cell lysate with MBS615326 at 1ug/ml.)
Rabbit ARFBP1 Polyclonal Antibody
ARFBP1 (HUWE1. HECT, UBA and WWE domain containing 1, ARF-binding protein 1, ARF-BP1, E3 ubiquitin-protein ligase HUWE1, HECT, UBA and WWE domain-containing protein 1, HectH9, HECTH9, Homologous to E6AP carboxyl terminus homologous protein 9, HSPC272, Ib7
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ARFBP1; Polyclonal Antibody; ARFBP1 (HUWE1. HECT; UBA and WWE domain containing 1; ARF-binding protein 1; ARF-BP1; E3 ubiquitin-protein ligase HUWE1; HECT; UBA and WWE domain-containing protein 1; HectH9; HECTH9; Homologous to E6AP carboxyl terminus homologous protein 9; HSPC272; Ib7; Anti -ARFBP1 (HUWE1. HECT; anti-ARFBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ARF-BP1. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-ARFBP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Dilution: Western Blot: 1-2ug/ml
Immunocytochemistry: 5ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunocytochemistry: 5ug/ml
Immunogen
19 amino acid peptide from near the carboxy terminus of human ARF-BP1 (GenBank accession no. NP_113584).
Positive Control
Daudi cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of MBS615326 in Daudi cell lysate with MBS615326 at 1ug/ml.)
Western Blot (WB)
(Western blot analysis of MBS615326 in Daudi cell lysate with MBS615326 at 1ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of ARF-BP1 in Daudi cells with MBS615326 at 5ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of ARF-BP1 in Daudi cells with MBS615326 at 5ug/ml.)
Related Product Information for anti-ARFBP1 antibody
The ARF tumor suppressor is a critical regulator of p53 stability (reviewed in 1). In addition to p53, ARF1 binds to other proteins such as MDM2 (2) and ARF-BP1, a large protein containing HECT, UBA and WWE motifs (3). ARFBP1 directly binds and ubiquitinates p53; this activity is
Product Categories/Family for anti-ARFBP1 antibody
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Product Notes
The ARFBP1 (Catalog #AAA615326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARFBP1 (HUWE1. HECT, UBA and WWE domain containing 1, ARF-binding protein 1, ARF-BP1, E3 ubiquitin-protein ligase HUWE1, HECT, UBA and WWE domain-containing protein 1, HectH9, HECTH9, Homologous to E6AP carboxyl terminus homologous protein 9, HSPC272, Ib7 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARFBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot and Immunocytochemistry. Dilution: Western Blot: 1-2ug/ml Immunocytochemistry: 5ug/ml. Researchers should empirically determine the suitability of the ARFBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARFBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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