Rabbit anti-Mouse, Rat HUWE1 Polyclonal Antibody | anti-HUWE1 antibody

RABBIT ANTI HUWE1

Cat. Num. AAA223881

• Gene Names: HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: HUWE1; Polyclonal Antibody; RABBIT ANTI HUWE1; anti-HUWE1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human HUWE1, a 148kDa DNA binding protein. ; HUWE1 is a novel E3 ubiquitin-protein ligase involved with mediating ubiquitination and subsequent proteasomal degradation of target proteins. ; HUWE1 is involved in apoptosis through catalyzation of the polyubiquitination and degradation of MCL1, ubiquitination of the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Research indicates that HUWE1 regulates the abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Total protein concentration 1.0mg/ml (varies by lot)
• Sequence Length: 4374

• Applicable Applications for anti-HUWE1 antibody: Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. ; Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Western Blotting: Minimum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A synthetic peptide corresponding to an 19 amino acid sequence from near the carboxy terminal of human HUWE1.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human HUWE1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human HUWE1 (MBS223881))

Related Product Information for anti-HUWE1 antibody

MBS223881 specifically recognises human HUWE1, a 148kDa DNA binding protein. HUWE1 is a novel E3 ubiquitin-protein ligase involved with mediating ubiquitination and subsequent proteasomal degradation of target proteins. HUWE1 is involved in apoptosis through catalyzation of the polyubiquitination and degradation of MCL1, ubiquitination of the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Research indicates that HUWE1 regulates the abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 61676188
• NCBI GeneID: 10075
• NCBI Accession #: NP_113584.3
• NCBI GenBank Nucleotide #: NM_031407.6
• UniProt Accession #: Q7Z6Z7; O15029; Q4G2Z2; Q5H961; Q6P4D0; Q8NG67; Q9BUI0; Q9HCJ4; Q9NSL6; Q9P0A9
• Molecular Weight: 481,018 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase HUWE1
• NCBI Official Synonym Full Names: HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: HUWE1
• NCBI Official Synonym Symbols: MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
• NCBI Protein Information: E3 ubiquitin-protein ligase HUWE1; ARF-binding protein 1; BJ-HCC-24 tumor antigen; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; URE-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9; large structure of UREB1; upstream regulatory element-binding protein 1
• UniProt Protein Name: E3 ubiquitin-protein ligase HUWE1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: HUWE1
• UniProt Synonym Gene Names: KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1; URE-binding protein 1
• UniProt Entry Name: HUWE1_HUMAN

NCBI Description

This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic mental retardation. [provided by RefSeq, Aug 2013]

Uniprot Description

HUWE1: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6. Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. Belongs to the TOM1/PTR1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein monoubiquitination; protein polyubiquitination; base-excision repair; histone ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; cell differentiation

Disease: Mental Retardation, X-linked, Syndromic, Turner Type